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大鼠惊吓模型尿液蛋白质组学分析

Urine proteomic analysis of rat startle model

摘要:惊吓带来的恐惧情绪是大脑产生的,我们试图用尿液蛋白质组学探索这种恐惧情绪能否在尿液中检测出来。我们采用天敌气味和声音刺激两种方式相结合共同建立了大鼠惊吓模型,在惊吓前后采集尿液样本并进行蛋白质组学分析。结果发现,惊吓后和惊吓前作比较,筛选出22个差异蛋白,这些差异蛋白富集到的生物学通路和神经递质运输、葡萄糖跨膜运输相关,这可能是惊吓引起的神经紧张状态表现。对每只大鼠进行了自身的前后对照分析发现,其中有1个蛋白被5只大鼠共同鉴定到,另外有19个蛋白被4只大鼠共同鉴定到,这些蛋白与神经、运动、代谢和血压的变化密切相关。其中包括谷氨酸半胱氨酸连接酶催化亚基及其调节亚基,谷氨酸半胱氨酸连接酶的功能可能和惊吓有关。这为研究惊吓的机制奠定了基础,为寻找治疗恐怖心理的药靶提供了新方法,同时充分说明了尿液的灵敏性,为尿液的探索开辟了新的领域。

英文摘要:The fear of startle is generated in the brain, and urine proteomics was used to explore whether this fear can be detected in the urine. The combination of natural enemy odor and sound stimulation were used to establish a rat model of startle. Urine samples were collected before and after startle and proteomic analysis was performed. The results showed that 22 differential proteins were identified after startle, and the biological pathways enriched in these differential proteins were related to neurotransmitter transport and glucose transmembrane transport, which may be the manifestations of nervous tension caused by startle. Before-after study in single rat was performed and found that there was one differential protein identified samely in five rats, in addition, 19 differential proteins were identified samely in four of five rats, and these proteins were associated with the change of nerve, motion, metabolism and blood pressure, which include catalytic subunits and regulatory subunits of glutamate-cysteine ligase, which realted to the function of startle. These results laid the foundation for the research of startle mechanism, and to find medicine for the treatment of psychological terror target provides a new method. At the same time, it fully illustrates the sensitivity of urine and opens up a new field for the exploration of urine.

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[V1] 2022-09-20 11:29:53 chinaXiv:202209.00131V1 下载全文
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