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1. chinaXiv:201707.00482 [pdf]

Effects of Cold Storage Environment Change on Antarctic Krill (Euphausia superba) Quality

Chen Shuai
Subjects: Geosciences >> Marine Sciences

In order to deal with the quality of Antarctic krill during the storage and transportation process caused by the failure of the supply system and the freezing equipment in the process of storage and circulation in the form of frozen products, the project team changed the refrigerated environment to the Antarctic The effect of krill quality was studied. When the ambient temperature increased from -20 ℃ to -12 ℃, -7 ℃, -3 ℃, -1.5 ℃, 0 ℃, 1 ℃, 2.5 ℃, 4 ℃, 6 ℃ , 8 ℃, 12.5 ℃, 16 ℃, the Antarctic krill samples for sensory evaluation, pH value, the meat rate and soup turbidity and other quality indicators of the determination. The results show that when the ambient temperature from -20 ℃ up to -1.5 ℃ process, the Antarctic krill soup after cooking is more clear, little change in turbidity, pH quickly increased from 7.60 to 7.85 Antarctic krill muscle elasticity began to change Poor, the sensory quality has begun to change from good to change; when the temperature from -1.5 ℃ to 4 ℃, the pH value slowly increased, there is a certain fluctuation, may be due to differences in the composition of the various components of the decision, Antarctic krill soup after the soup turbidity quickly increased from 0.5 to 0.7Brix maximum, sensory quality is still at a moderate level; when the temperature rose to 16 ℃, the pH has risen to 8.05, Antarctic krill Sensory quality from the change into a bad, this time the Antarctic krill has been unable to eat, only as a feed or other use.

submitted time 2017-07-17 Hits11430Downloads1272 Comment 0

2. chinaXiv:201707.00481 [pdf]

The Study on Flow Velocity Measurement of Antarctic Krill Trawl Model Experiment in North Bay of South China Sea

Chen Shuai
Subjects: Geosciences >> Marine Sciences

From August 25 to 29, 2014, the project team carried out the experiment of Antarctic krill trawl in the Beihai Bay of the South China Sea. In order to understand the flow field of the network model in the course of the experiment, it is necessary to record the speed of the ship and to grasp the flow field of the ocean. Therefore, the ocean velocity is measured during the experiment. The flow rate in this experiment was measured using an acoustic Doppler flowmeter (Vectrino Plus, Nortek, Norway). In order to compensate for the flow rate error caused by ship drift, the drift condition of the ship was also measured by the positioning device (Snapdragon MSM8274AB, Qualcomm, USA) used in the flow rate measurement. The results show that the actual velocity of the target sea area is in the range of 0.06-0.49 m / s and the direction is 216.17-351.70. And compared with the previous research, the influencing factors were analyzed. This study proves that it is feasible to use point Doppler flowmeter for velocity study in trawl model experiment.

submitted time 2017-07-17 Hits11690Downloads1279 Comment 0

3. chinaXiv:201605.01326 [pdf]

Interaction of NS2 with AIMP2 Facilitates the Switch from Ubiquitination to SUMOylation of M1 in Influenza A Virus-Infected Cells

Gao, Shijuan; Li, Jiandong; Song, Liping; Sheng, Chunjie; Liu, Dong; Jiang, Wei; Chen, Shuai; Huang, Wenlin; Wu, Jiaoxiang; Yao, Chen; Chen, Huiming; Liu, Ran-Yi; Chen, Shuai; Huang, Wenlin; Huang, Wenlin; Teng, Yan
Subjects: Biology >> Biophysics

Influenza A viruses (IAVs) rely on host factors to support their life cycle, as viral proteins hijack or interact with cellular proteins to execute their functions. Identification and understanding of these factors would increase our knowledge of the molecular mechanisms manipulated by the viruses. In this study, we searched for novel binding partners of the influenza A virus NS2 protein, the nuclear export protein responsible for overcoming host range restriction, by a yeast two-hybrid screening assay and glutathione S-transferase-pulldown and coimmunoprecipitation assays and identified AIMP2, a potent tumor suppressor that usually functions to regulate protein stability, as one of the major NS2-binding candidates. We found that the presence of NS2 protected AIMP2 from ubiquitin-mediated degradation in NS2-transfected cells and AIMP2 functioned as a positive regulator of IAV replication. Interestingly, AIMP2 had no significant effect on NS2 but enhanced the stability of the matrix protein M1. Further, we provide evidence that AIMP2 recruitment switches the modification of M1 from ubiquitination to SUMOylation, which occurs on the same attachment site (K242) on M1 and thereby promotes M1-mediated viral ribonucleoprotein complex nuclear export to increase viral replication. Collectively, our results reveal a new mechanism of AIMP2 mediation of influenza virus replication. IMPORTANCE Although the ubiquitination of M1 during IAV infection has been observed, the precise modification site and the molecular consequences of this modification remain obscure. Here, we demonstrate for the first time that ubiquitin and SUMO compete for the same lysine (K242) on M1 and the interaction of NS2 with AIMP2 facilitates the switch of the M1 modification from ubiquitination to SUMOylation, thus increasing viral replication.

submitted time 2016-05-11 Hits920Downloads496 Comment 0

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