Current Location:home > Browse

1. chinaXiv:201605.01738 [pdf]

Crystal structure of cyclic nucleotide-binding-like protein from Brucella abortus

He, Zheng; Gao, Yuan; Li, Xuemei; Zhang, Xuejun C.; He, Zheng; Dong, Jing; Ke, Yuehua; Chen, Zeliang
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology

The cyclic nucleotide-binding (CNB)-like protein (CNB-L) from Brucella abortus shares sequence homology with CNB domain-containing proteins. We determined the crystal structure of CNB-L at 2.0 angstrom resolution in the absence of its C-terminal helix and nucleotide. The 3D structure of CNB-L is in a twofold symmetric form. Each protomer shows high structure similarity to that of cGMP-binding domain-containing proteins, and likely mimics their nucleotide-free conformation. A key residue, Glu17, mediates the dimerization and prevents binding of cNMP to the canonical ligand-pocket. The structurally observed dimer of CNB-L is stable in solution, and thus is likely to be biologically relevant. (C) 2015 Elsevier Inc. All rights reserved.

submitted time 2016-05-15 Hits10133Downloads1401 Comment 0

  [1 Pages/ 1 Totals]