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1. chinaXiv:202002.00070 [pdf]

Towards an effective mRNA vaccine against 2019-nCoV: demonstration of virus-like particles expressed from a modified mRNA cocktail

Xia, Jia; Lu, Guoliang; Lu, Jing; Zhang, Jiahui; Feng, Lin; Wang, Bing; Yu, Hang; Xu, Yingjie; Lin, Jinzhong
Subjects: Biology >> Virology

Frequent outbreaks of coronavirus make the development of an effective vaccine imperative. Recently, vaccines based on in-vitro transcribed messenger RNA (mRNA) have shown great potential. The streamlined manufacturing of mRNA molecules, combined with the superior flexibility in the antigen screening, greatly accelerates the development process. When using an mRNA platform to develop a vaccine, initial antigen choice plays a crucial role in determining the final efficacy and safety of the vaccine. Furthermore, mRNA sequences that encode antigens require extensive optimization to ensure highly efficient and sustained expression. Our ongoing efforts to develop an effective mRNA vaccine against 2019-nCoV place emphasis on the virus-like particles (VLPs) as the presenting antigen. At the same time, our second fast track uses mRNA to express the receptor-binding domain of the spike protein(S-RBD). After extensive optimization, an mRNA cocktail containing three genes is able to produce 2019-nCoV virus-like particles highly similar to the native 2019-nCoV. Meanwhile, an mRNA vaccine candidate expressing S-RBD is being tested in mice for its immunogenicity. We will next compare both the efficacy and the safety of the two mRNA vaccines based on S-RBD and VLPs, respectively.

submitted time 2020-02-25 Hits14913Downloads2890 Comment 0

2. chinaXiv:201812.00009 [pdf]

The posterior cranial portion of the earliest known Tetrapodomorph Tungsenia paradoxa and the early evolution of tetrapodomorph endocrania

LU Jing; YOUNG Gavin; HU Yu-Zhi; QIAO Tuo; ZHU Min
Subjects: Biology >> Other Disciplines of Biology

Abstract Here the posterior cranial portion of the tetrapodomorph Tungsenia from the Lower Devonian (Pragian, ~409 million years ago) of Yunnan, southwest China, is reported for the first time. The pattern of posterior skull roof and the morphology of the otoccipital region of the neurocranium are described in detail, providing precious insight into the combination of cranial characters of the earliest known tetrapodomorph to date. The posterior cranium of Tungseniadisplays a mosaic of features previously linked either to basal dipnomorphs such as Youngolepis(e.g., the well-developed subjugular ridge, the strong adotic process, and the poorly developed fossa bridgei) or to typical tetrapodomorphs (e.g., the lateral dorsal aortae commenced from the median dorsal aorta postcranially). The independent ventral arcual plate is also found in the advanced tetrapodomorph Eusthenopteron. The new endocranial material of Tungsenia further fills in the morphological gap between Tetrapodomorpha (tetrapod lineage) and Dipnomorpha (lungfish lineage) and unveils the sequence of character acquisition during the initial diversification of the tetrapod lineage. The new phylogenetic analysis strongly supports the basalmost position of Tungsenia amongst the tetrapod lineage.

submitted time 2018-12-04 From cooperative journals:《古脊椎动物学报》 Hits4530Downloads1322 Comment 0

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