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1. chinaXiv:202002.00070 [pdf]

Towards an effective mRNA vaccine against 2019-nCoV: demonstration of virus-like particles expressed from a modified mRNA cocktail

Xia, Jia; Lu, Guoliang; Lu, Jing; Zhang, Jiahui; Feng, Lin; Wang, Bing; Yu, Hang; Xu, Yingjie; Lin, Jinzhong
Subjects: Biology >> Virology

Frequent outbreaks of coronavirus make the development of an effective vaccine imperative. Recently, vaccines based on in-vitro transcribed messenger RNA (mRNA) have shown great potential. The streamlined manufacturing of mRNA molecules, combined with the superior flexibility in the antigen screening, greatly accelerates the development process. When using an mRNA platform to develop a vaccine, initial antigen choice plays a crucial role in determining the final efficacy and safety of the vaccine. Furthermore, mRNA sequences that encode antigens require extensive optimization to ensure highly efficient and sustained expression. Our ongoing efforts to develop an effective mRNA vaccine against 2019-nCoV place emphasis on the virus-like particles (VLPs) as the presenting antigen. At the same time, our second fast track uses mRNA to express the receptor-binding domain of the spike protein(S-RBD). After extensive optimization, an mRNA cocktail containing three genes is able to produce 2019-nCoV virus-like particles highly similar to the native 2019-nCoV. Meanwhile, an mRNA vaccine candidate expressing S-RBD is being tested in mice for its immunogenicity. We will next compare both the efficacy and the safety of the two mRNA vaccines based on S-RBD and VLPs, respectively.

submitted time 2020-02-25 Hits14911Downloads2889 Comment 0

2. chinaXiv:201705.00723 [pdf]

Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia

Wang, Lan; Zhou, Guang-Biao; Liu, Ping; Song, Jun-Hong; Liang, Yang; Yan, Xiao-Jing; Xu, Fang; Wang, Bing-Shun; Mao, Jian-Hua; Shen, Zhi-Xiang
Subjects: Biology >> Biomedical Laboratory Science

To enhance therapeutic efficacy and reduce adverse effects, practitioners of traditional Chinese medicine (TCM) prescribe a combination of plant species/minerals, called formulae, based on clinical experience. Nearly 100,000 formulae have been recorded, but the working mechanisms of most remain unknown. In trying to address the possible beneficial effects of formulae with current biomedical approaches, we use Realgar-Indigo naturalis formula (RIF), which has been proven to be very effective in treating human acute promyelocytic leukemia (APL) as a model. The main components of RIF are realgar, Indigo naturalis, and Salvia miltiorrhiza, with tetraarsenic tetrasulfide (A), indirubin (I), and tanshinone IIA (T) as major active ingredients, respectively. Here, we report that the ATI combination yields synergy in the treatment of a murine APL model in vivo and in the induction of APL cell differentiation in vitro. ATI causes intensified ubiquitination/degradation of promyelocytic leukemia (PML)-retinoic acid receptor #1; (RAR#1;) oncoprotein, stronger reprogramming of myeloid differentiation regulators, and enhanced G1/G0 arrest in APL cells through hitting multiple targets compared with the effects of mono- or biagents. Furthermore, ATI intensifies the expression of Aquaglyceroporin 9 and facilitates the transportation of A into APL cells, which in turn enhances A-mediated PML-RAR#1; degradation and therapeutic efficacy. Our data also indicate A as the principal component of the formula, whereas T and I serve as adjuvant ingredients. We therefore suggest that dissecting the mode of action of clinically effective formulae at the molecular, cellular, and organism levels may be a good strategy in exploring the value of traditional medicine.

submitted time 2017-05-10 Hits3356Downloads1072 Comment 0

3. chinaXiv:201705.00431 [pdf]

Nanostructures of Stereocomplex Polylactide in Poly[l-lactide] Doped with Poly[d-lactide]

Huang, W [Huang, Wei][ 1,4 ]; Luo, XZ [Luo, Xian-Zi][ 1 ]; Wang, BJ [Wang, Bing-Jie][ 1 ]; Wei, WF [Wei, Wen-Feng][ 1 ]; Chen, P [Chen, Peng][ 1 ]; Gu, Q [Gu, Qun][ 1 ]; de Vos, S [de Vos, Sicco][ 2 ]; Wang, RY [Wang, Ru-Yin][ 3 ]; Joziasse, CAP [Joziasse, Cornelis A. P.][ 2 ]

Nanostructures of stereocomplex polylactide [sc-PLA] are obtained and studied in poly[l-lactide] [PLLA] doped with a low amount of poly[d-lactide] [PDLA] during successive melt-quenching, extrusion, spinning, and drawing processes corresponding to quiesce

submitted time 2017-05-02 Hits472Downloads290 Comment 0

4. chinaXiv:201705.00328 [pdf]

Synthesis and properties of poly[3-hydroxybutyrate-co-3-hydroxyvalerate]/chitin nanocrystals composite scaffolds for tissue engineering

Li, HY [Li, Hu-Yan][ 1,2 ]; Li, H [Li, He][ 2 ]; Wang, BJ [Wang, Bing-Jie][ 2 ]; Gu, Q [Gu, Qun][ 3 ]; Jiang, ZQ [Jiang, Zhi-Qiang][ 3 ]; Wu, XD [Wu, Xue-Dong][ 2 ]

Poly[3-hydroxybutyrate-co-3-hydroxyvalerate] [PHBV]/chitin nanocrystals [CNC] composite scaffolds were synthesized by the salt leaching and thermally induced phase separation [TIPS] technique. The scaffolds have porous structures with macro-pores [100-300

submitted time 2017-05-02 Hits472Downloads284 Comment 0

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