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1. chinaXiv:201705.00601 [pdf]

Synthesis of poly[butylene terephthalate]-poly[tetramethylene glycol] copolymers using terephthalic acid as starting material: A comparation between two synthetic strategies

Zhang, JW [Zhang, Jun-wu][ 1 ]; Liu, F [Liu, Fei][ 1 ]; Wang, JG [Wang, Jing-gang][ 1 ]; Na, HN [Na, Hai-ning][ 1 ]; Zhu, J [Zhu, Jin][ 1 ]

Poly[butylene terephthalate]-poly[tetramethylene glycol] [PBT-PTMG] copolymer is prepared with terephthalic acid [PTA] rather than its dimethyl ester [DMT] as starting material by a two-step melt polycondensation. This process includes the synthesis of PB

submitted time 2017-05-02 Hits896Downloads485 Comment 0

2. chinaXiv:201605.01740 [pdf]

Aberrantly upregulated TRAP1 is required for tumorigenesis of breast cancer

Zhang, Bo; Wei, Peng; Hao, Junfeng; Zhao, Lijing; Zhang, Fenglin; Wei, Taotao; Wang, Jing; Huang, Zhen; Wei, Peng; Liu, Ying; Tu, Yaping
Subjects: Biology >> Biophysics >> Oncology

Tumor necrosis factor receptor-associated protein 1 (TRAP1) is abnormally expressed in many cancers. In this study, we showed that TRAP1 is aberrantly upregulated in breast tumors compared to control tissues. TRAP1 knockdown downregulates mitochondrial aerobic respiratory, sensitizes cells to lethal stimuli, and inhibited tumor growth in MDA-MB-231 and MCF-7 breast cancer cells in vivo. TRAP1 overexpression, however, enhances the capacity to cope with stress conditions. These evidences suggested that TRAP1 is required for tumorigenesis. We also found that TRAP1 regulates the mitochondrial morphology. Relatively lower TRAP1 levels are associated with the rod-shaped mitochondrial phenotype in invasive and metastatic MDA-MB-231 breast cancer cells; on the contrary, higher TRAP1 levels are associated with the tubular network-shaped mitochondrial phenotype in non-invasive MCF-7 cells. Interestingly, the expression of TRAP1 in human breast cancer specimens inversely correlates with tumor grade. Overexpression of TRAP1 in MDA-MB-231 cells causes mitochondrial fusion, triggers mitochondria to form tubular networks, and suppresses cell migration and invasion in vitro and in vivo. These data link TRAP1-regulated mitochondrial dynamics and function with tumorigenesis of breast cancer and suggested that TRAP1 may therefore be a potential target for breast cancer drug development.

submitted time 2016-05-15 Hits3689Downloads1379 Comment 0

3. chinaXiv:201605.01726 [pdf]

The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold

Wan, Zhengpeng; Chen, Xiangjun; Chen, Yingjia; Wang, Jing; Cao, Yiyun; Liu, Wanli; Chen, Haodong; Wang, Fei; Tang, Zhuo; Ji, Qinghua; Lou, Jizhong
Subjects: Biology >> Biophysics >> Biology

B lymphocytes use B cell receptors (BCRs) to sense the physical features of the antigens. However, the sensitivity and threshold for the activation of BCRs resulting from the stimulation by mechanical forces are unknown. Here, we addressed this question using a double-stranded DNA-based tension gauge tether system serving as a predefined mechanical force gauge ranging from 12 to 56 pN. We observed that IgM-BCR activation is dependent on mechanical forces and exhibits a multi-threshold effect. In contrast, the activation of isotype-switched IgG- or IgE-BCR only requires a low threshold of less than 12 pN, providing an explanation for their rapid activation in response to antigen stimulation. Mechanistically, we found that the cytoplasmic tail of the IgG-BCR heavy chain is both required and sufficient to account for the low mechanical force threshold. These results defined the mechanical force sensitivity and threshold that are required to activate different isotyped BCRs.

submitted time 2016-05-15 Hits2192Downloads1278 Comment 0

4. chinaXiv:201605.01406 [pdf]

The Role of the Peptidyl-Prolyl cis/trans Isomerase Pin1 in The Occurrence and Development of Alzheimer's Disease

Wang Jing-Zhang; Hou Hai; Li Xue-Mei
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology

Pin1 is the only known cis-trans isomerase that recognizes pThr/pSer-Pro in proteins, relevant to the pathogenesis of Alzheimer' s disease (AD). Pin1 regulates the structures and functions of some molecules that are related to AD, inhibits the main AD pathological characteristics such as neurofibrillary tangles (NFTs), senile plaques (SPs), and cerebral amyloid angiopathy (CAA), promotes the differentiation of neural progenitor cells (NPCs) to neurons, and to some extent prevents the occurrence and development of AD. Meanwhile, Pin1 dysfunction in vivo may be involved in the pathogenesis of AD. Nevertheless, whether Pin1 could be a therapeutic target for the prevention and treatment of AD still needs to be verified clinically. Considering of the poor efficacy of AD medicines that target each single molecule in brain, the "combined multiple-target medicine" focusing on Pin1 and other related molecules may be a therapeutic strategy for AD in the future.

submitted time 2016-05-12 Hits1160Downloads654 Comment 0

5. chinaXiv:201605.01351 [pdf]

CD163+CD14+macrophages, a potential immune biomarker for malignant pleural effusion

Wang, Fei; Yang, Li; Gao, Qun; Huang, Lan; Zhang, Yi; Wang, Fei; Zhang, Yi; Gao, Qun; Wang, Liping; Zhang, Yi; Wang, Jing; Wang, Shengdian; Zhang, Bin
Subjects: Biology >> Biophysics >> Oncology

Malignant pleural effusion (MPE) is a common complication caused by malignant diseases. However, subjectivity, poor sensitivity, and substantial false-negative rates of cytology assay hamper accurate MPE diagnosis. The aim of this study was to assess whether CD163+CD14+ tumor-associated macrophages (TAMs) could be used as a biomarker for enabling sensitive and specific MPE diagnosis. Pleural effusion samples and peripheral blood samples were collected from 50 MPE patients and 50 non-malignant pleural effusion (NMPE) patients, respectively. Flow cytometry was performed to analyze cell phenotypes, and RT-qPCR was used to detect cytokine expression in these monocytes and macrophages. A blinded validation study (n = 40) was subsequently performed to confirm the significance of CD163+CD14+ TAMs in MPE diagnosis. Student's t test, rank sum test, and receiver operating characteristic curve analysis were used for statistical analysis. Notably, CD163+CD14+ cell frequency in MPE was remarkably higher than that in NMPE (P < 0.001). In a blinded validation study, a sensitivity of 78.9 % and a specificity of 100 % were obtained with CD163+CD14+ TAMs as a MPE biomarker. In total (n = 140), by using a cutoff level of 3.65 %, CD163+CD14+ cells had a sensitivity of 81.2 % and a specificity of 100 % for MPE diagnosis. Notably, MPE diagnosis by estimating CD163+CD14+ cells in pleural effusion could be obtained one week earlier than that obtained by cytological examination. CD163+CD14+ macrophages could be potentially used as an immune diagnostic marker for MPE and has better assay sensitivity than that of cytological analysis.

submitted time 2016-05-11 Hits3488Downloads1240 Comment 0

6. chinaXiv:201605.01262 [pdf]

Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci

Dong, Qiuhua; Dong, Qiuhua; Wang, Jing; Yang, Hang; Wei, Cuihua; Yu, Junping; Zhang, Yun; Huang, Yanling; Wei, Hongping; Zhang, Xian-En
Subjects: Biology >> Biophysics

Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysin PlyV12. The results showed that the chimeric lysin Ply187N-V12C had not only lytic activity similar to Ply187N against staphylococcal strains but also extended its lytic activity to streptococci and enterococci, such as Streptococcusdysgalactiae, Streptococcus agalactiae, Streptococcus pyogenes, Enterococcus faecium and Enterococcus faecalis, which Ply187N could not lyse. Our work demonstrated that generating novel chimeric lysins with an extended lytic spectrum was feasible through fusing a catalytic domain with a cell-binding domain from lysins with lytic spectra across multiple genera.

submitted time 2016-05-11 Hits1123Downloads662 Comment 0

7. chinaXiv:201605.00705 [pdf]

Transformable Peptide Nanocarriers for Expeditious Drug Release and Effective Cancer Therapy via Cancer-Associated Fibroblast Activation

Ji, Tianjiao; Zhao, Ying; Ding, Yanping; Wang, Jing; Zhao, Ruifang; Lang, Jiayan; Qin, Hao; Shi, Jian; Nie, Guangjun; Zhao, Yuliang; Liu, Xiaoman; Tao, Ning; Qin, Zhihai;
Subjects: Biology >> Biophysics

A novel cleavable amphiphilic peptide (CAP) was designed to be specifically responsive to fibroblast activation protein- (FAP-), a protease specifically expressed on the surface of cancer-associated fibroblasts. The CAP self-assembled into fiber-like nanostructures in solution, while the presence of hydrophobic chemotherapeutic drugs readily transformed the assemblies into drug-loaded spherical nanoparticles. The disassembly of these nanoparticles (CAP-NPs) upon FAP- cleavage resulted in rapid and efficient release of the encapsulated drugs specifically at tumor sites. This Transformers-like drug delivery strategy could allow them to disrupt the stromal barrier and enhance local drug accumulation. Therapeutic results suggested that drug-loaded CAP-NPs hold promising tumor specificity and therapeutic efficacy for various solid tumor models, confirming its potential utility and versatility in antitumor therapy.

submitted time 2016-05-05 Hits1191Downloads624 Comment 0

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