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1. chinaXiv:201711.02415 [pdf]

Visualization?and?Quantification?of?Browning?Using?a?Ucp1-2A-Luciferase Knock-in Mouse Model

Mao, LF; Nie, BM; Nie, T; Hui, XY; Gao, XF; Lin, XL; Liu, X; Xu, Y; Tang, XF; Yuan, R; Li, K; Li, P; Ding, K; Wang, Y; Xu, AM; Fei, J; Han, WP; Liu, PT; Madsen, L; Kristiansen, K
Subjects: Biology >> Ecology

Both mammals and adult humans possess classic brown adipocytes and beige adipocytes, and the amount and activity?of?these adipocytes are considered key factors in combating obesity and its associated metabolic diseases. Uncoupling protein 1 (Ucp1) is the functional marker?of?both brown and beige adipocytes. To facilitate?a?reliable, easy, and sensitive measurement?of?Ucp1 expression both in vivo and in vitro, we generated?a?Ucp1-2A-luciferase knock-in mouse by deleting the stop codon for the mouse Ucp1 gene and replacing it with?a?2A peptide. This peptide was followed by the luciferase coding sequence to recapitulate the expression?of?the Ucp1 gene at the transcriptional and translational levels. With this mouse, we discovered?a?cold-sensitive brown/beige adipose depot underneath the skin?of?the ears, which we named uBAT. Because?of?the sensitivity and high dynamic range?of?luciferase activity, the Ucp1-2A-luciferase mouse is useful for both in vitro quantitative determination and in vivo?visualization?of?nonshivering thermogenesis. With the use?of?this model, we identified and characterized axitinib, an oral small-molecule tyrosine kinase inhibitor, as an effective?browning?agent.

submitted time 2017-11-17 Hits1118Downloads647 Comment 0

2. chinaXiv:201711.02422 [pdf]

Fumarylacetoacetate?Hydrolase?Knock-out?Rabbit?Model?for?Hereditary?Tyrosinemia?Type?1

Li, L?(Li, Li); Zhang, QJ?(Zhang, Quanjun); Yang, HQ?(Yang, Huaqiang); Zou, QJ?(Zou, Qingjian); Lai, CD?(Lai, Chengdan); Jiang, F?(Jiang, Fei); Zhao, P?(Zhao, Ping); Luo, ZW?(Luo, Zhiwei); Yang, JY?(Yang, Jiayin); Chen, Q?(Chen, Qian); Wang, Y?(Wang, Yan); Newsome, PN?(Newsome, Philip N.); Frampton, J?(Frampton, Jon); Maxwell, PH?(Maxwell, Patrick H.); Li, WJ?(Li, Wenjuan); Chen, SH?(Chen, Shuhan); Wang, DY?(Wang, Dongye); Siu, TS?(Siu, Tak-Shing); Tam, S?(Tam, Sidney); Tse, HF?(Tse, Hung-Fat)
Subjects: Biology >> Ecology

From the (a)CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, and Guangzhou Medical University, Guangzhou 511436, China, (b)CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, (c)Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China, (d)Cardiology Division, Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, China, (e)Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, The University of Hong Kong and Guangzhou Institutes of Biomedicine and Health, Hong Kong SAR, China, (f)Department of Ophthalmology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China, (g)State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Guangdong Provincial Research Center?for?Liver Fibrosis, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital and (h)Biomedical Research Center, Southern Medical University, Guangzhou 510515, China, (i)Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, (j)National Institute?for?Health Research (NIHR) Birmingham Liver Biomedical Research Unit and Centre?for?Liver Research, and (k)Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom, (l)Cambridge Institute?for?Medical Research, Wellcome Trust/Medical Research Council (MRC) Building, Cambridge CB2 0XY, United Kingdom, mDepartment of Clinical Biochemistry Unit, Queen Mary Hospital, Hong Kong SAR, China, (n)Department of Medicine, University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, Guangdong, China, and (o)Laboratory of Metabolism and Cell Fate, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, Guangdong, China

submitted time 2017-11-17 Hits1079Downloads594 Comment 0

3. chinaXiv:201711.02433 [pdf]

Absence of Appl2 sensitizes endotoxin shock through activation of PI3K-Akt pathway

Mao, LF?(Mao, Liufeng); Lin, WH?(Lin, Wanhua); Nie, T?(Nie, Tao); Hui, XY?(Hui, Xiaoyan); Gao, XF?(Gao, Xuefei); Li, K?(Li, Kuai); Ding, MX?(Ding, Mengxiao); Tang, XF?(Tang, Xiaofeng); Li, P?(Li, Peng); Wang, Y?(Wang, Yu); Xu, AM?(Xu, Aimin); Liu, PT?(Liu, Pentao); Wu, DH?(Wu, Donghai)
Subjects: Biology >> Ecology

Background: The adapter proteins Appl1 (adaptor protein containing pleckstrin homology domain, phosphotyrosine domain, and leucine zipper motif 1) and Appl2 are highly homologous and involved in several signaling pathways. While previous studies have shown that Appl1 plays a pivotal role in adiponectin signaling and insulin secretion, the physiological functions of Appl2 are largely unknown. Results: In the present study, the role of Appl2 in sepsis shock was investigated by using Appl2 knockout (KO) mice. When challenged with lipopolysaccharides (LPS), Appl2 KO mice exhibited more severe symptoms of endotoxin shock, accompanied by increased production of proinflammatory cytokines. In comparison with the wild-type control, deletion of Appl2 led to higher levels of TNF-alpha and IL-1 beta in primary macrophages. In addition, phosphorylation of Akt and its downstream effector NF-kappa B was significantly enhanced. By co-immunoprecipitation, we found that Appl2 and Appl1 interacted with each other and formed a complex with PI3K regulatory subunit p85 alpha, which is an upstream regulator of Akt. Consistent with these results, deletion of Appl1 in macrophages exhibited characteristics of reduced Akt activation and decreased the production of TNF alpha and IL-1 beta when challenged by LPS. Conclusions: Results of the present study demonstrated that Appl2 is a critical negative regulator of innate immune response via inhibition of PI3K/Akt/NF-kappa B signaling pathway by forming a complex with Appl1 and PI3K.Conclusions: Results of the present study demonstrated that Appl2 is a critical negative regulator of innate immune response via inhibition of PI3K/Akt/NF-kappa B signaling pathway by forming a complex with Appl1 and PI3K.

submitted time 2017-11-17 Hits1140Downloads627 Comment 0

4. chinaXiv:201711.00047 [pdf]

Investigation on Inlet Recirculation Characteristics of Double Suction Centrifugal Compressor with Unsymmetrical Inlet

Yang, C., ; Wang, Y. J.,; Lao, D. Z., ; Tong, D., ; Wei, L. Y. ; Liu, Y. X.
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc.

This paper focuses on the flow characteristic and local resistance of non-Newtonian power law fluid in a curved 90 bend pipe with circular cross-sections, which are widely used in industrial applications. By employing numerical simulation and theoretical analysis the properties of the flow and local resistance of power law fluid under different working conditions are obtained. To explore the change rule the experiment is carried out by changing the Reynolds number, the wall roughness and different diameter ratio of elbow pipe. The variation of the local resistance coefficient with the Reynolds number, the diameter ratio and the wall roughness is presented comprehensively in the paper. The results show that the local resistance force coefficient hardly changes with Reynolds number of the power law fluid; the wall roughness has a significant impact on the local resistance coefficient. As the pipe wall roughness increasing, the coefficient of local resistance force will increase. The main reason of the influence of the roughness on the local resistance coefficient is the increase of the eddy current region in the power law fluid flow, which increases the kinetic energy dissipation of the main flow. This paper provides theoretical and numerical methods to understand the local resistance property of non-Newtonian power law fluid in elbow pipes.

submitted time 2017-11-02 From cooperative journals:《热科学学报》 Hits763Downloads355 Comment 0

5. chinaXiv:201705.00436 [pdf]

Enhanced catalytic degradation of 4-NP using a superhydrophilic PVDF membrane decorated with Au nanoparticles

Wu, ZY [Wu, Ziyang][ 1,2 ]; Lin, HB [Lin, Haibo][ 1 ]; Wang, YZ [Wang, Yunze][ 1 ]; Yu, XM [Yu, Xuemin][ 1,2 ]; Li, JL [Li, Jinglong][ 1 ]; Xiong, Z [Xiong, Zhu][ 1 ]; Wang, Y [Wang, Yi][ 1 ]; Huang, YJ [Huang, Youju][ 1 ]; Chen, T [Chen, Tao][ 1 ]; Liu, F [Liu, Fu][ 1 ]
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc.

Poly(vinylidene fluoride) (PVDF) membranes have been widely applied to treat wastewater, however, the removal of toxic aromatic phenolic compounds remains a technical challenge due to the serious adsorption fouling and difficult degradation. Herein, we aimed to design a superhydrophilic PVDF membrane decorated with Au nanoparticles, which enhanced the rapid degradation of p-nitrophenol (4-NP). The superhydrophilic PVDF membrane with a micro/nano structured surface was decorated with Au nanoparticles via poly(dopamine) (PDA) as a spacer. The influences of membrane affinity (e.g. Hydrophilic Membrane (HM), micro/nano structured superhydrophilic membrane (MSiM), and micro/ nano structured superhydrophobic membrane (MSoM)) on PDA deposition and the subsequent Au decoration were comprehensively investigated. The synthesized Au nanoparticles were characterized using transmission electron microscopy (TEM) and UV-vis absorption spectra. The morphology and composition was evaluated using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Static catalytic experiments demonstrated that MSiM degraded over 90% of 4-NP in 5 minutes with a kinetic reaction rate constant of 47.84 10 2 min 1 and high stability over 6 cycles. A membrane catalytic reactor (MCR) was designed to realize the continuous catalytic degradation of 4-NP with a kinetic reaction rate constant of 7 10 2 min 1.

submitted time 2017-05-02 Hits810Downloads516 Comment 0

6. chinaXiv:201705.00380 [pdf]

Unveiling the adsorption mechanism of zeolitic imidazolate framework-8 with high efficiency for removal of copper ions from aqueous solutions

Zhang, YJ [Zhang, Yujie][ 1,2,3 ]; Xie, ZQ [Xie, Zhiqiang][ 4 ]; Wang, ZQ [Wang, Zhuqing][ 1,2,3,5 ]; Feng, XH [Feng, Xuhui][ 6 ]; Wang, Y [Wang, Ying][ 4 ]; Wu, AG [Wu, Aiguo][ 1,2,3 ]
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc.

Among the heavy metal ions, copper(II) can cause eye and liver damage at high uptake. The existence of copper ions (Cu2+) even with an ultralow concentration of less than 0.1 μg g?1 can be toxic to living organisms. Thus, it is highly desirable to develop efficient adsorbents to remove Cu2+ from aqueous solu- tions. In this work, without any surface functionalization or pretreatment, a water-stable zeolitic imidazo- late framework (ZIF-8) synthesized at room temperature is directly used as a highly efficient adsorbent for removal of copper ions from aqueous solutions. To experimentally unveil the adsorption mechanism of Cu2+ by using ZIF-8, we explore various effects from a series of important factors, such as pH value, contact time, temperature and initial Cu2+ concentration. As a result, ZIF-8 nanocrystals demonstrate an unexpected high adsorption capacity of Cu2+ and high removal efficiency for both high and low concen- trations of Cu2+ from water. Moreover, ZIF-8 nanocrystals possess fast kinetics for removing Cu2+ with the adsorption time of less than 30 min. In addition, the pH of the solution ranging from 3 to 6 shows little effect on the adsorption of Cu2+ by ZIF-8. The adsorption mechanism is proposed for the first time and systematically verified by various characterization techniques, such as TEM, FTIR, XPS, XRD and SEM.

submitted time 2017-05-02 Hits805Downloads551 Comment 0

7. chinaXiv:201703.01019 [pdf]

Hypoxia dysregulates the production of adiponectin and plasminogen activator inhibitor-1 independent of reactive oxygen species in adipocytes

Chen, BY; Lam, KSL; Wang, Y; Wu, DH; Lam, MC; Shen, JG; Wong, LC; Hoo, RLC; Zhang, JL; Xu, AM
Subjects: Biology >> Biomedical Laboratory Science

Low plasma levels of adiponectin (hypoadiponectinemia) and elevated circulating concentrations of plasminogen activator inhibitor (PAI)-1 are causally associated with obesity-related insulin resistance and cardiovascular disease. However, the mechanism that mediates the aberrant production of these two adipokines in obesity remains poorly understood. In this study, we investigated the effects of hypoxia and reactive oxygen species (ROS) on production of adiponectin and PAI-1 in 3T3-L1 adipocytes. Quantitative PCR and immunoassays showed that ambient hypoxia markedly suppressed adiponectin mRNA expression and its protein secretion, and increased PAI-1 production in mature adipocytes. Dimethyloxallyl glycine, a stabilizer of hypoxia-inducible factor 1a (HIF-1a), mimicked the hypoxiamediated modulations of these two adipokines. Hypoxia caused a modest elevation of ROS in adipocytes. However, ablation of intracellular ROS by antioxidants failed to alleviate hypoxia-induced aberrant production of adiponectin and PAI-1. On the other hand, teantioxidants could reverse hydrogen peroxide (H2O2)-induced dysregulation of adiponectin and PAI-1 production. H2O2 treatment decreased the expression levels of peroxisome proliferator-activated receptor gamma (PPARc) and CCAAT/enhancer binding protein (C/EBPa), but had no effect on HIF-1a, whereas hypoxia stabilized HIF-1a and decreased expression of C/EBPa, but not PPARc. Taken together, these data suggest that hypoxia and ROS decrease adiponectin production and augment PAI-1 expression in adipocytes via distinct signaling pathways. These effects may contribute to hypoadiponectinemia and elevated PAI-1 levels in obesity, type 2 diabetes,and cardiovascular diseases.

submitted time 2017-03-30 Hits2256Downloads926 Comment 0

8. chinaXiv:201612.00373 [pdf]

Prospect of a very long baseline neutrino oscillation experiment: HIPA to Beijing

H2B-1,VSG; Chen,H; Ding,L; He,J; Kuang,H; Lu,Y; Ma,Y; Shan,L; Shen,C; Wang,Y; Yang,C; Zhang,X; Zhu,Q; Qing,C; Xiong,Z; Yang,JM; Zhang,Z; Chen,J; Ye,Y; Lee,S
Subjects:

We discuss the prospects of a very long baseline neutrino oscillation experiment from HIPA to Beijing. The current understanding of neutrino oscillations, both theoretically and experimentally, are summarized. The figure of merits for interested physics m

submitted time 2016-12-28 Hits1620Downloads827 Comment 0

9. chinaXiv:201612.00372 [pdf]

Probing neutrino oscillations jointly in long and very long baseline experiments

H2B-4,VSG; Wang,Y; Whisnant,K; Xiong,Z; Yang,JM; Young,B
Subjects:

We examine the prospects of making a joint analysis of neutrino oscillation at two baselines with neutrino superbeams. Assuming narrow band superbeams and a 100 kt water Cerenkov calorimeter, we calculate the event rates and sensitivities to the matter ef

submitted time 2016-12-28 Hits1275Downloads714 Comment 0

10. chinaXiv:201605.01578 [pdf]

ENERGY DISSIPATION PROCESSES IN SOLAR WIND TURBULENCE

Wang, Y.; Wei, E. S.; Feng, X. S.; Xu, X. J.; Zhang, J.; Sun, T. R.; Zuo, P. B.
Subjects: Geosciences >> Space Physics

Turbulence is a chaotic flow regime filled by irregular flows. The dissipation of turbulence is a fundamental problem in the realm of physics. Theoretically, dissipation ultimately cannot be achieved without collisions, and so how turbulent kinetic energy is dissipated in the nearly collisionless solar wind is a challenging problem. Wave particle interactions and magnetic reconnection (MR) are two possible dissipation mechanisms, but which mechanism dominates is still a controversial topic. Here we analyze the dissipation region scaling around a solar wind MR region. We find that the MR region shows unique multifractal scaling in the dissipation range, while the ambient solar wind turbulence reveals a monofractal dissipation process for most of the time. These results provide the first observational evidences for intermittent multifractal dissipation region scaling around a MR site, and they also have significant implications for the fundamental energy dissipation process.

submitted time 2016-05-12 Hits749Downloads432 Comment 0

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