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1. chinaXiv:201811.00111 [pdf]

Microbial adaptation to long-term N supply prevents large responses in N dynamics and N losses of a subtropical forest

Han, Xiaodge; Shen, Weijun; Zhang, Jinbo; Muller, Christoph
Subjects: Biology >> Biochemistry

Atmospherically-deposited nitrogen (N) can stimulate complex soil N metabolisms and accumulations over time. Whether long-term (decadal) N deposition effects on soil N transformations and functional microbes differ from the short-term (annual) effects have rarely been assessed. Here we conducted a laboratory 15N tracing study with soil samples from a short-term (one year) N addition site and a long-term (12 years) site in a subtropical forest. The effects of simulated N deposition on soil N2O emissions, N transformation rates and microbial nitrifying and denitrifying genes were determined. Our results showed that: (1) long-term N addition did not change soil N2O fluxes significantly in comparison to the short-term N addition. Denitrification, heterotrophic nitrification and autotrophic nitrification contributed 53%, 23% and 18% to total N2O emissions, respectively. (2) Autotrophic nitrification was the dominant N transformation process, except for the high-N treatment at the long-term site. The magnitude of soil N transformation rates was significantly different among N addition treatments but not between short- and long-term N addition sites. However, long-term N addition changed the responses of specific N transformation rates to N addition markedly, especially for the rates of nitrification, organic N mineralization to NH4+, NO3- immobilization and dissimilatory NO3- reduction to NH4+ (DNRA). (3) Responses of ammonia oxidizing archaea and bacteria (AOA and AOB) were stronger than denitrifying N2O-producers (nirK) and denitrifying N2O-reducers (nosZ) at the long-term site compared to the short-term site. (4) The close correlations among N2O flux, functional genes and soil properties observed at the short-term site was weakened at the long-term site, posing a decreased risk for N losses in the acid subtropical forest soils. There is evidence for an adaptation of functional microbial communities to the prevailing soil conditions and in response to long-term natural and anthropogenic N depositions.

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