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1. chinaXiv:202104.00023 [pdf]

动脉粥样硬化ApoE-/-小鼠模型尿液蛋白质组学变化探究

华元瑞; 孟文书; 魏静; 刘永涛; 高友鹤
Subjects: Biology >> Biochemistry

心血管疾病是当今世界第一大致死因素,动脉粥样硬化是其重要病理基础,其早期诊断具有重要意义。尿液更利于积累和反应机体生理状态的变化且不受到稳态机制的调节,是疾病早期生物标志物的良好来源。本文通过高脂饮食诱导ApoE-/-小鼠5个月构建动脉粥样硬化动物模型,选取七个时间点收集实验组及正常饮食对照组c57BL/6小鼠尿液样品,使用蛋白质组学分析方法,进行自身对照和组间对照。自身对照结果表明高脂饮食1周前后尿液蛋白质组差异显著,同时多种差异蛋白被报道过与动脉粥样硬化相关或可作为其生物标志物。组间对照结果表明差异蛋白GO分析得出的生物学过程能够对应疾病发生发展的进程。两种对照方式得到的差异性化学修饰也多被报道与该疾病进程相关。实验证明了尿液蛋白质组学有敏锐监测机体变化的潜力,为寻找动脉粥样硬化早期生物标志物提供了可能。

submitted time 2021-04-08 Hits0Downloads0 Comment 0

2. chinaXiv:202104.00022 [pdf]

老年人的消极交往与心理健康:实证、理论与展望

徐潞杰; 张镇
Subjects: Psychology >> Applied Psychology

积极交往和消极交往分别会对老年人的心理健康产生有利和不利影响,然而,探讨消极交往的负面作用及其机制的研究相对较少。目前研究主要涉及消极交往的类型(社会隔离、丧偶或离婚)、来源(配偶、子女或朋友)及强度,并探讨感知评估、自尊的中介作用及性别、婚姻、受教育水平等变量的调节作用。社会情感选择理论、优劣势整合理论、社会交换理论及镜中我理论在一定范围内对消极交往的负面作用做出了合理解释。采取“原谅”或“认知疏远”的应对策略能有效缓解消极交往的负面影响。未来研究应进一步考察不同文化背景及社会网络背景下消极交往对老年人心理健康的影响,同时加强对消极交往的理论研究和线上研究,并关注相关的干预研究。

submitted time 2021-04-07 Hits102Downloads47 Comment 0

3. chinaXiv:202104.00021 [pdf]

“One-to-three” droplet generation-based digital microfluidics for parallel immunoassay.docx

Kai, Jin; Chenxuan, Hu; Siyi, Hu; Jinhua, Li; Hanbin, Ma
Subjects: Biology >> Bioengineering

In digital microfluidics, droplet generation is the fun, as the basis for a series of droplet manipulations including mixing, separation, dispensing, etc., has always been the focus in the field of digital microfluidics. Currently, the performance of DMF-based droplet splitting is highly influenced by the ratio of electrode size and the gap between the upper and lower plates. Here we propose a "one to three" droplet splitting method. In "one to three" spitting method, a pair of opposite electrowetting forces are applied to the droplet. As the electrowetting force is applied on the splitting position, a middle child droplet and two mother liquid droplets generate. The aspect ratio of child droplet is close to 1:2. "One to three" spitting method is then applied to magnetic beads immunoassay. Magnetic beads are retained in child droplets under small magnetic force with high magnetic bead washing efficiency. Simultaneous detection of five B-type natriuretic peptide analyte samples are performed on a single chip within 10 minutes. Correlation coefficient (R2) of the calibration curve was 0.9942, and the detection limit was less than 5pg/mL. This unique method of digital microfluidic droplet splitting has great potential in other application fields.

submitted time 2021-04-07 Hits81Downloads34 Comment 0

4. chinaXiv:202104.00020 [pdf]

Bacterial and viral therapies of cancer background, mechanism and perspective

董庆霖
Subjects: Medicine, Pharmacy >> Preclinical Medicine

Bacterial and viral therapies of cancer are highly promising, yet their mechanisms are incompletely understood, hindering their improvement and application. In this paper, We (1) review briefly the genesis and progress of bacterial and viral therapies of cancer, (2) compare and evaluate the proposed mechanisms of bacterial and viral therapies and present the unifying mechanism that bacterial and viral therapies of cancer mainly take advantage of the immune response of cancer cells to bacteria and viruses and the ability of host immune system to recognize and eliminate the responsive cancer cells, and (3) provide a perspective on the exploitation of non-human and non-animal bacteria and viruses, particularly protist-infecting bacteria and viruses and bacterial virus (bacteriophage), for cancer therapy.

submitted time 2021-04-06 Hits94Downloads39 Comment 0

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