广藿香原生质体制备、培养与融合技术优化研究

Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-06-22 Cooperative journals: 《广西植物》

严寒静1,2；李磊1 ；张宏意1,2；何梦玲1,2

Abstract： For establishing a high efficient and stable protoplast culture and fusion technology system, the protoplasts obtained from Pogostemon cablin’s callus, were studied as the material for enzymatic hydrolysis conditions of protoplast preparation, and on this base, the effects of culture methods, cell density, hormone types and concentrations on the protoplast culture were studied. Screening range of fusion cells were determined by measuring the diameter of fusion products. The effects of PEG concentration, cell density, incubation time and amount of fusion fluid on the fusion rate of protoplasts were studied by single factor analysis. The results showed that the yield of protoplast was higher with enzymolysis temperature about 23-28 ℃ and the vitality of protoplast reached the highest value at 25 ℃. The optimal pH for enzymatically hydrolyzing was 5.8. Four methods, shallow layer of liquid, double layer of solid liquid, agarose embedding and sodium alginate embedding, were be used to culture protoplast of P. cablin, the protoplasts with sodium alginate embedding began to divide earlier and got the higher division frequency and plating efficiency.

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