Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-07-18 Cooperative journals: 《广西植物》
Abstract： Actinidia chinensis ‘Yangshi Jinhong 50’is a fine Actinidia chinensis Planch cultivar of yellow-flesh andred-heart,which favored bypeopleandknown as rich nourishment.In orderto establishtherapidandefficient propagation system in virtro, this study used stem with axillary bud of Actinidia chinensis ‘Yangshi Jinhong 50’as the explant, and used tissue culture to study suitable explants sterilization method, best plant growth regulator combination in Actinidia chinensis ‘Yangshi Jinhong 50’. This study founded two kinds of in vitro regeneration modes. Mode I : the axillary buds of stem with axillary bud were induced directly; Mode II : the callus was induced first, and then the adventitious buds were induced. The results showed that the explants best sterilization method was 75% alcohol 30 s+ 15% Ca(ClO)2 5 min+ 0.1% mercuric chloride 8 min; In Mode I, the plant growth substances combination of MS+ 4.0 mg·L-1 6-BA+ 0.1 mg·L-1 NAAfor axillary buds germination had the best the induction rate, which could reach 91.67%; In Mode II, callus rate of inducing stems bottom was more than 80%, the optimal medium that induced stems bottom callus to generate adventitious buds was MS+ 3.0 mg·L-1 6-BA+ 0.3 mg·L-1 NAA, germination rate reach 83.33%; the best plant growth substances combination in tufted bud culture was MS+ 4.0 mg·L-1 6-BA+ 0.4 mg·L-1 NAA, multiplication coefficient as high as 6.14; the best plant growth substances combination in rooting culture was 1/2 MS+ 0.9 mg·L-1 IBA, getting the differentiation of adventitious root about 15 d and complete plant 45 d, rooting rate reach 72.64%, average rooting number reach 25.67, average root length reach 2.67 cm, average plant height reach 3.08 cm. After the seedlings rooted, transplanted the seedlings to the substrate, theseedlings could reach the 96% survival rate in the substrate with the garden soil∶sand = 1∶1. Through this study, the micropropagation system in vitro of Actinidia chinensis ‘Yangshi Jinhong 50’ was established, which provides the basis for the research of genetic transformation in Actinidia chinensis Planch, at the same time, the establishment regeneration system provides a good technical supportforseedlingmicropropagationandfactoryinActinidiachinensis‘YangshiJinhong50’.
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