Study on the Secondary metabolites of Cladosporium sp. SCSIO41206, a symbiotic epiphytic fungus of coral in Gulf of Tonkin

Subjects: Geosciences >> Marine Sciences submitted time 2023-08-15

王梦芹 帖青青 李云秋 刘永宏 杨斌

Abstract： Based on the OSMAC strategy, this paper explores and investigates the structurally novel compounds in the secondary metabolites of Cladosporium sp. SCSIO41206, a coral-associated fungus from the Beibu Gulf. The Secondary metabolites of the bacterium were isolated and purified by various chromatographic techniques, and the compounds were identified by NMR, MS and data comparison with relevant literature. The research results showed that this fungus produced richer metabolites when cultured in the Potato Glucose Water (PBD) Liquid Culture Medium and incubated at 26°C for 30 days. Through isolation and identification, twelve known monomeric compounds were obtained, namely3-isobutylhexahydropyrrolo(1,2-a)pyrazine-1,4-dione(1);3-benzyl-7-hydroxyhexahydropyrrolo(1,2-a)pyrazine-1,4-dione(2);cyclo(L-proline-L-4-hydroxyproline)dipeptide(3);cyclo(L-proline-glycine)dipeptide(4);cyclo(D)-Pro-(D)-Ile(5);cyclo(phenylalanine-glycine)dipeptide(6);cyclo-(4-S-hydroxy-R-proline-R-isoleucine)(7);N-phenethylacetamide(8);N-hydroxy-2-(hydroxyimino)-4-methylpentanamide(9);quinolactacin A1(10); quinolactacin A2(11); Dibutylterephthalate(12). Compounds 10-12 were isolated for the first time from Cladosporium sp., a branch fungus obtained from the Beibu Gulf coral. Compounds 2、3、4、8、10、11 and 12 showed weak inhibitory activity against acetylcholinesterase.

Peer Review Status:Awaiting Review

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