分类: 物理学 >> 核物理学 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要: Many environmental factors can cause DNA damage, such as radiation, heat, oxygen free radical, etc., which can induce mutation during DNA replication. Meanwhile, DNA molecules are subjected to various mechanical forces in numerous biological processes. However, it is unknown whether the mechanical force would induce DNA damage and introduce mutation during DNA replication. With the combination of single-molecule manipulation based on atomic force microscopy (AFM), single molecular polymerase chain reaction (SM-PCR) and Sangers sequencing, we investigated the effect of mechanical force on DNA. The results show that mechanical force can cause DNA damage and induce DNA mutation during amplification.
分类: 天文学 >> 天文学 提交时间: 2024-05-24 合作期刊: 《Research in Astronomy and Astrophysics》
摘要: In this study, we present a catalog of molecular clumps extracted from 13CO (J = 1 − 0) emission data of the Milky Way Imaging Scroll Painting (MWISP) project. The data covers the inner Milky Way within the longitude range 10° ≤ l ≤ 20° and the latitude strip of . The workflow for the extraction of clumps, namely Facet-SS-3D-Clump, consists of two parts: the identification of clump candidates and their verification. First, Facet-SS-3D-Clump employs FacetClumps to identify clump candidates. Subsequently, high-confidence clumps are obtained by cross-matching with the clumps detected by other algorithms, such as dendrogram. Second, these high-confidence clumps are used as prior knowledge to train a semi-supervised deep clustering approach, SS-3D-Clump, which is applied to verify clump candidates detected by FacetClumps, providing confidence levels for the molecular clumps. Finally, the catalog comprising 18,757 molecular clumps was obtained using Facet-SS-3D-Clump, and the catalog is 90% complete above 37 K km s−1. We observe a significant deviation of the mean Galactic latitude for clumps within ∣b∣ ≤ 2° from the midplane, with . We found that 82.3% of the dust clumps correspond to 13CO clumps by matching with Herschel infrared dust clumps. In the future, Facet-SS-3D-Clump will be applied to detect 13CO clumps in the entire MWISP data.