Subjects: Biology >> Bioengineering submitted time 2017-09-20
Abstract： Chromatography has been playing an important role in bioseparation. As one of the main components of chromatography, the structure and properties of chromatographic media have great effects on purification results. Among them, capacity is one of the most important properties to determine chromatographic media’s purification effects, and how to improve the capacity is one of the key points having been paid close attention to in bioseparation. This paper is based on high-capacity agarose-based chromatographic media, with hydrophobic and immobilized metal ion affinity chromatographic media being tailor-made, respectively. For hydrophobic chromatographic media, various lengths of spacer arm were introduced with a controllable hydrophobic property, and the media was used in CHO-rHBsAg purification. rHBsAg binding capacity was greatly improved by this media containing spacer-arms, and rHBsAg recovery was more than 90% and the purification factor was 65.8, both higher than the results from traditional chromatographic media. For immobilized metal ion affinity chromatographic media, dextran molecules were grafted with manipulation and its capacity of His-tagged recombinant proteins was enhanced by 1.5 times than that of traditional media. This novel tailor-made high-capacity chromatographic media is promising for large scale purification of recombinant proteins.
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