Subjects: Biology >> Virology submitted time 2020-02-14
Abstract： Abstract: In 2019, the 2019 novel Coronavirus (2019-nCoV) has caused the pneumonia outbreak in Wuhan (a city of China). In our previous study, the analytical results showed that both 2019-nCoV and SARS coronavirus belongs to Betacoronavirus subgroup B (BB coronavirus), but have large differences. The most important finding was that the alternative translation of Nankai CDS could produce more than 17 putative proteins, which may be responsible for the host adaption. The genotyping of 13 viruses using the 17 putative proteins revealed the high mutation rate and diversity of betacoronavirus. The present study for the first time reported a very important mutation in the Spike (S) proteins of BB coronavirus. By this mutation, 2019-nCoV acquired a cleavage site for furin enzyme, which is not present in the S proteins of all other BB coronavirus (e.g. SARS coronavirus) except the Mouse Hepatitis coronavirus (MHV). This mutation may increase the efficiency of virus infection into cells, making 2019-nCoV has significantly stronger transmissibility than SARS coronavirus. Because of this mutation, the packing mechanism of the 2019-nCoV may be changed to being more similar to those of MHV, HIV, Ebola virus (EBoV) and some avian influenza viruses, other than those of all other BB coronavirus (e.g. SARS coronavirus) except the Mouse Hepatitis coronavirus (MHV). In addition, we unexpectedly found that some avian influenza viruses acquired a cleavage site for furin enzyme by mutation as 2019-nCoV. Further studies of this mutation will help to reveal the stronger transmissibility of 2019-nCoV and lay foundations for vaccine development and drug design of, but not limited to 2019-nCoV.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Ecology submitted time 2017-03-08 Cooperative journals: 《生态学报》
Abstract：以羽茅（Achnatherum sibiricum）为材料，采用室内生物测定法研究感染不同内生真菌的羽茅浸提液对大针茅（Stipa grandis）种子萌发和幼苗生长的化感作用，并采用气相色谱-质谱（GC-MS）联用技术对羽茅浸提液中的化学成分进行分析。结果表明：（1）内生真菌感染可缓解宿主羽茅对群落优势种大针茅的化感作用；（2）内生真菌感染对宿主羽茅化感作用的影响与内生真菌种类有关，具体表现为相比于未染菌羽茅，感染Epichloë gansuensis可显著缓解宿主羽茅对大针茅的抑制作用，而感染Neotyphodium sibiricum、Neotyphodium gansuensis有缓解羽茅对大针茅化感作用的趋势，但未达到显著影响；（3）羽茅浸提液中含有2，4-二叔丁基苯酚、邻苯二甲酸、邻苯二甲酸二丁酯和硬脂酸甲酯等潜在化感物质，这4种物质相对含量的差异可能是不同内生真菌感染状态的羽茅产生不同化感效应的主要原因；（4）对羽茅浸提液中4种潜在化感物质的化感作用进行验证实验，结果表明，2，4-二叔丁基苯酚对大针茅的化感作用基本表现为低浓度无影响，高浓度抑制，邻苯二甲酸二丁酯的化感作用表现为“低促高抑”的效应趋势，而邻苯二甲酸和硬脂酸甲酯对大针茅均表现出“剂量效应”的化感作用趋势，即4种潜在化感物质在高浓度时均可显著抑制大针茅的种子萌发和幼苗生长。
Hits
Hits
Downloads
Comment