Subjects: Physics >> Interdisciplinary Physics and Related Areas of Science and Technology submitted time 2016-05-08
Abstract: Single-molecule experiments revealed that爐he爎eplication rate爋f燭7燚NA爌olymerase爃as燼爌eak value at爐ension爋f燼bout 5.5 pN. However,爐he爉echanism leading to this peak has only been partially investigated. Here, we present燼爐wo-state model to investigate爐he爀ffect爋f爉echanical爐ension爋n燚NApolymerase燼ctivity.燭he爉odel consists爋f爌olymerase燼nd exonuclease.燭he爌olymerase's kinetic pathway has been simplified into Michaelis-Menten formwith爐wo steps. One is爐he燾onformational change from "open" to "close".燭he爋ther is爐he燽ase-stacking.燭he爎esults are in good agreement爓ith爀xperimental observations. We also predict that爐he爐ension爋n爐emplate is beneficial to爐he爁idelity爋f燚NA爎eplication.
Peer Review Status:Awaiting Review