Strategy of tuning gene expression ratio in prokaryotic cell from perspective of noise andcorrelation

Subjects: Physics >> Interdisciplinary Physics and Related Areas of Science and Technology submitted time 2016-05-08

rui li liufang xu hualinshi

Abstract： Genes are organized into operons爄n爌rocaryote, and these genes爄n爋ne operon generally have related functions. However, genes爄n爐he same operon are usually not equally expressed, and the爎atio爊eeds to be fine-tuned for specific functions. We examine the difference爋f爂ene爀xpression爊oise燼nd燾orrelationwhen爐uning爐he爀xpression爈evel at the transcriptional or translational level爄n燼 bicistronic operon driven by a constitutive or a two-state promoter. We get analytic results for the爊oise燼nd燾orrelation爋f爂ene爀xpression爈evels, which is confirmed by our stochastic simulations. Both the爊oise燼nd the燾orrelation爋fgene爀xpressions爄n燼n operon with a two-state promoter are higher than爄n燼n operon with a constitutive promoter. Premature termination爋f爉RNA induced by transcription terminator爄n爐he intergenic region or changing translation rates can tune the protein爎atio燼t the transcriptional level or at the translational level. We find that爂ene爀xpression燾orrelation燽etween promoter-proximal and promoter-distal genes at the protein level decreases as termination increases.營ncontrast, changing translation rates爄n爐he normal range almost does not alter the燾orrelation. This explains why the translation rate is a key factor爋fmodulating爂ene爀xpressions爄n燼n operon. Our results can be useful to understand the relationship between the operon structure and the biological function爋fa爂ene爊etwork, and also may help爄n爏ynthetic biology design.�

Peer Review Status:Awaiting Review

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