Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-06-15 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To observe the effect of different time windows and interventions on skin pressure ulcers and ischemiareperfusion (I/R) injury in rats. Methods Sixty-eight SD rats were randomly divided into blank control group (n=4) and model group (n=64). The rats in the model group were randomly divided into group A (n=32) without intervention and group B (n= 32) with post-conditioning. The degree of skin compression, neutrophil infiltration and serum levels of free radicals were observed in the rats after compression for 2, 4, 6, and 8 h (8 rats at each time point). Results A significant difference was found in the severity of skin damage among the control group, group A, and group B (P=0.001), and the injury was milder in group B than in group A. Severe skin lesions occurred in 2 rats after skin compression for 6 h, as compared with 6 after compression for 8 h (P=0.043), but in none of the rats after compression for 2 or 4. Seventeen rats in group B and 15 in group A showed grade 1 neutrophil infiltration in the skin lesions, and 8 rats in group B and 10 in group A showed grade II neutrophil infiltration (P=0.002). Neutrophil infiltration was the mildest in rats with a 2-h compression, and exacerbated progressively and significantly as the compression time extended (P=0.027). With the prolongation of the intervention time, the rats in both groups A and B showed decreased SOD and increased MDA and NO levels, and overall the I/R injury was milder in 2- and 4-h compression groups than in 6- and 8-h compression groups. The level of serum SOD was significantly higher and MDA and NO levels were significantly higher in group B than in group A (P<0.05). Conclusion Ischemic post-conditioning can relieve I/R injury in acute pressure ulcer in rats. The effective time window for intervention is within 6 h of ischemia, and the effect of ischemic postconditioning is optimal within 2 h. Ischemic post-conditioning can alleviate free radical injury and inflammation caused by I/R injury.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-06-14 Cooperative journals: 《协和医学杂志》
Abstract： IgG4-related disease (IRD) is a newly defined multi-organ involved autoimmune disease only about 10 years ago. It is characterized by elevated serum IgG4, storiform fibrosis and massive infiltration of IgG4 positive plasma cells in involved organs. Biliary tract is one of the most common involved organs.IgG4-related sclerosing cholangitis (IRSC) is the clinical entity of IRD in biliary tract.It always has the similar clinical symptoms to biliary-pancreatic malignancy, such as localized biliary wall thickness, dilation of bile duct and obstructive jaundice. It is sensitive to steroids with satisfactory prognosis, which does not warrant surgical treatment. However, recent years, the authors met several cases who underwent operation due to bile duct stricture and obstructive jaundice. However, the final pathological examinations reported that they were IRSC. After reviewing these medical cases, the authors found IRSC was seldom considered before surgery. As well, there were some other cases of biliary malignancy with elevated serum IgG4 which were misdiagnosed to be IRS, leading to missing the best operative time. Therefore, to widely broadcast the knowledge of IRSC and especially to deepen the understanding of IRSC for the hepatic-biliary-pancreatic surgeons are of great clinical importance.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To observe the effect of different time windows and interventions on skin pressure ulcers and ischemiareperfusion (I/R) injury in rats. Methods Sixty-eight SD rats were randomly divided into blank control group (n=4) and model group (n=64). The rats in the model group were randomly divided into group A(n=32) without intervention and group B (n= 32) with post-conditioning. The degree of skin compression, neutrophil infiltration and serum levels of free radicals were observed in the rats after compression for 2, 4, 6, and 8 h (8 rats at each time point). ResultsAsignificant difference was found in the severity of skin damage among the control group, groupA, and group B (P=0.001), and the injury was milder in group B thaningroupA.Severeskinlesionsoccurredin2ratsafterskincompressionfor6h,ascomparedwith6aftercompressionfor 8 h (P=0.043), but in none of the rats after compression for 2 or 4. Seventeen rats in group B and 15 in groupAshowed grade 1 neutrophil infiltration in the skin lesions, and 8 rats in group B and 10 in group Ashowed grade II neutrophil infiltration (P= 0.002). Neutrophil infiltration was the mildest in rats with a 2-h compression, and exacerbated progressively and significantly as the compression time extended (P=0.027). With the prolongation of the intervention time, the rats in both groups A and B showed decreased SOD and increased MDA and NO levels, and overall the I/R injury was milder in 2- and 4-h compression groups than in 6- and 8-h compression groups. The level of serum SOD was significantly higher and MDAand NO levels were significantly higher in group B than in groupA(P<0.05). Conclusion Ischemic post-conditioning can relieve I/Rinjury in acute pressure ulcer in rats. The effective time window for intervention is within 6 h of ischemia, and the effect of ischemic postconditioning is optimal within 2 h. Ischemic post-conditioning can alleviate free radical injury and inflammation caused by I/R injury.
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