Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-06-15 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of propofol on myelin basic protein (MBP) expression in oligodendrocytes of SD rats at different developmental stages. Methods This study was conducted in 3-, 7-, 14- and 21-day-old SD rats (40 in each age group). In each group, the rats were randomized equally into control group and experimental group, and in the control group, the rats received an intraperitoneal injection of 25 mg/kg medium-long-chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium (at the initial dose of 25 mg/ kg) in the same manner. The transcriptional levels of MBP and caspase-3 in the brain tissues were detected by qRT-PCR, and the protein expression of MBP was with Western blotting and immunehistochemistry. Results Compared with those in the control groups, the expression of MBP mRNA was significantly down-regulated while caspase-3 mRNA was up-regulated in 3-, 7- and 14-day-old rats in the experimental groups (P<0.05). The protein expression of MBP in 7- and 14-day-old rats was significantly decreased in the experimental groups compared with the control groups (P<0.05). The expression of caspase-3 mRNA or MBP protein in 21-day-old rats showed no significant difference between the two groups (P>0.05). Conclusion Propofol can down-regulate the expression of MBP at both the mRNA and protein levels in SD rats, especially in those at 7 and 14 days of age.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of propofol on myelin basic protein (MBP) expression in oligodendrocytes of SD rats at different developmental stages. Methods This study was conducted in 3-, 7-, 14- and 21-day-old SD rats (40 in each age group). In each group, the rats were randomized equally into control group and experimental group, and in the control group, the rats received an intraperitoneal injection of 25 mg/kg medium-long-chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium (at the initial dose of 25 mg/ kg) in the same manner. The transcriptional levels of MBP and caspase-3 in the brain tissues were detected by qRT-PCR, and the protein expression of MBP was with Western blotting and immunehistochemistry. Results Compared with those in the control groups, the expression of MBP mRNA was significantly down-regulated while caspase-3 mRNA was up-regulated in 3-, 7- and 14-day-old rats in the experimental groups (P<0.05). The protein expression of MBP in 7- and 14-day-old rats was significantly decreased in the experimental groups compared with the control groups (P<0.05). The expression of caspase-3 mRNA or MBP protein in 21-day-old rats showed no significant difference between the two groups (P>0.05). Conclusion Propofolcandown-regulatetheexpressionofMBPatboththemRNAandproteinlevelsinSDrats,especiallyinthoseat7and 14daysofage.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of propofol on cell invasion and expressions of aquaporin-3 (APQ-3) and matrix metalloproteinase-9 (MMP-9) in human lung adenocarcinoma cancerA549 cells. MethodA549 cells were treated with propofol at the concentrations of 25, 50, and 100 μmol/L for 12 or 24 h. RT-PCR was used to detect the effect of propofol on AQP-3 mRNA level in A549 cells, and the effects of propofol treatments for 24 h on AQP-3 and MMP-9 protein expression and the invasive ability of A549 cells were assessed with Western blotting and Transwell assay, respectively. Results Compared with the control cells, the cells treated with 25, 50, and 100 μmol/L propofol showed a obvious inhibition of AQP-3 mRNA expression, with inhibition rates ranging from 0.19 to 0.65 in cells with a 12-h treatment and from 0.13 to 0.41 in cells treated for 24 h; 100 μmol/L propofol treatment for 24 h produced the strongest inhibitory effect (0.13±0.035, P<0.05). AQP-3 protein expression in cells treated with 25, 50, and 100 μmol/L propofol for 24 h (0.91±0.009, 0.60±0.020, and 0.57±0.006, respectively) and MMP-9 protein expression in cells treated with 50 and 100 μmol/L propofol for 24 h (0.65±0.006 and 0.46±0.021, respectively) were significantly lower than those in the control cells (P<0.05). Treatment with 25, 50, and 100 μmol/L propofol for 24 significantly lowered the number of invading cells (122.55±17.20, 96.33±5.82, and 74.33±2.85, respectively) compared withthecontrolgroup (199.33±23.88,P<0.05).ConclusionTreatmentwith50and100μmol/Lpropofolinhibitscellinvasionby down-regulatingtheexpressionofAQP-3andMMP-9inA549cells.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To explore the effect of propofol on H19 expression, migration and invasion of human breast cancer MDA-MB-231 cells in vitro. Methods MDA-MB-231 cells were randomly divided into 5 groups for treatment with basal medium, DMSO, or propofol at concentrations of 25, 50, and 100 μmol/L. H19 expression of the treated cells was assessed with RT-PCR, and the changes of cell motility, migration and invasion were evaluated with wound-healing assay and Transwell assays. Results Treatment of the cells with 25, 50, and 100 μmol/L propofol for 24 h down-regulated H19 by 17.83%, 37.50% and63.67%(P<0.05),andsuppressedcellmotility by 13.46%, 36.54%and 46.17%(P<0.05),cellmigration by 27.93%, 57.90%and 76.51% (P<0.05), and cell invasion by 25.72%, 53.32% and 81.43% (P<0.05), respectively. Conclusion Propofol-induced cell migrationandinvasionsuppressionarepartiallymediatedbydown-regulatingH19inMDA-MB-231cellsinvitro.
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