Subjects: Biology >> Biochemistry submitted time 2024-01-19
Abstract： Zinc is an essential element for maintaining normal physiological function in living organisms. In this study, 82 mg/kg·d zinc gluconate (equivalent to 11.7 mg/kg·d zinc) was intragastrically administered to rats for 4 days, and the urine proteome of rats before and after short-term intragastric administration of zinc gluconate was compared and analyzed. Many differential proteins have been reported to be zinc related, such as mucin-2 (MUC-2) (14 times before compared with after gavage, p = 0.005) and transthyretin (3.9 times after gavage compared with before gavage, p = 0.0004). Biological processes enriched in differential proteins (e.g., regulation of apoptosis process, immune system process, etc.), molecular functions (e.g., calcium binding, copper binding, signaling receptor activity, etc.), KEGG pathways (e.g., complement and coagulation cascades, PI3K-Akt signaling pathway, etc.) showed correlation with zinc. In this study, we explore the overall effect of zinc on the body from the perspective of urine proteomics, which is helpful to deeply understand the biological function of zinc and broaden the application potential of urine proteomics.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-18
Abstract： Calcium is an essential element for maintaining the normal physiological function of organisms. In this study, 3225 mg/kg · d calcium gluconate (equivalent to 300 mg/kg · d calcium) was intragastrically administered to rats for 4 days, and the urine proteome of rats was analyzed. Many differential proteins have been reported to be calcium related, such as Regucalcin (2.6 times higher after gavage than before gavage, p = 0.022), transmembrane protein 132A (8.2 times higher after gavage than before gavage, p = 0.009), creatine kinase (17.5 times higher before gavage than after gavage, p = 0.006), and claudin-3 (13.3 times higher before gavage than after gavage, p = 0.037). Differential protein enriched KEGG pathways included calcium signaling pathways, and biological processes and molecular functions also showed correlation with calcium. In this study, from the perspective of urine proteomics to explore the overall impact of calcium on the body, it is helpful to deeply understand the biological function of calcium and broaden the application potential of urine proteomics.
Peer Review Status:Awaiting Review
Subjects: Biology >> Neurobiology submitted time 2024-01-09
Abstract： Video game addiction manifests as an escalating enthusiasm and uncontrolled use of digital games, yet there are no objective indicators for gaming addiction. This study employed mass spectrometry proteomics to analyze the proteomic differences in the urine of adolescents addicted to gaming compared to those who do not play video games. The study included 10 adolescents addicted to gaming and 9 non-gaming adolescents as a control group. The results showed that there were 125 significantly different proteins between the two groups. Among these, 11 proteins have been reported to change in the body after the intake of psychotropic drugs and are associated with addiction: Calmodulin, ATP synthase subunit alpha, ATP synthase subunit beta, Acid ceramidase, Tomoregulin-2, Calcitonin, Apolipoprotein E, Glyceraldehyde-3-phosphate dehydrogenase, Heat shock protein beta-1, CD63 antigen, Ephrin type-B receptor 4, Tomoregulin-2. Additionally, several proteins were found to interact with pathways related to addiction: Dickkopf-related protein 3, Nicastrin, Leucine-rich repeat neuronal protein 4, Cerebellin-4. Enriched biological pathways discovered include those related to nitric oxide synthase, amphetamine addiction, and numerous calcium ion pathways, all of which are associated with addiction. Moreover, through the analysis of differentially expressed proteins, we speculated about some proteins not yet fully studied, which might play a significant role in the mechanisms of addiction: Protein kinase C and casein kinase substrate in neurons protein, Cysteine-rich motor neuron 1 protein, Bone morphogenetic protein receptor type-2, Immunoglobulin superfamily member 8. In the analysis of urinary proteins in adolescents addicted to online gaming, we identified several proteins that have previously been reported in studies of drug addiction.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2024-01-04
Abstract： Iron is an essential trace element to maintain the normal physiological function of organisms. No studies have investigated the overall effect of iron on the body from the perspective of urine proteome. In this study, the urine proteome of rats before and after short-term intragastric administration of polysaccharide-iron complex (28mg/kg·d iron, which is equivalent to the dose of anemia prevention in adults) was compared and analyzed by using two analysis methods: individual comparison and group comparison. Many different proteins were reported to be related to iron, including 2', 3' -cyclic nucleotide 3' -phosphodiesterase (CNPase) (7.7 times higher than that after gavage, p=0.0039), p38 (14.5 times higher than that before gavage, p=0.003), etc. In the individual comparison, Hepcidin was up-regulated in 4 rats simultaneously. The biological processes of differential protein enrichment include carbohydrate metabolism, iron ion reaction, apoptosis regulation, hematopoietic progenitor cell differentiation, etc. Molecular functions (e.g., complement binding, hemoglobin binding, etc.), KEGG pathways (e.g., complement and coagulation cascade, cholesterol metabolism, malaria, etc.) have also been shown to be associated with iron. This study contributes to the in-depth understanding of the biological function of iron from the perspective of urine proteomics, and provides a new research perspective for the prevention, diagnosis, treatment and monitoring of iron-related disorders.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2023-05-05
Abstract： Urine proteome can reflect how short-term changes in growth and development of animal organisms and whether short-term developmental effects on urinary protein need to be considered when performing urine marker studies using animal models built with faster growing periods? In this study, urine samples were collected from 10 Wistar rats aged 6-8 weeks 3 and 6 days apart and analyzed using a non-labeled quantitative proteomics technique with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The results showed that urine proteome could sensitively reflect the changes of short-term growth and development in rats. For example, comparing the urine proteome of Day0 and Day6, 195 differential proteins could be identified after screening (FC ≥ 1.5 or ≤ 0.67, P < 0.05), and verified by randomization, the average number of randomly generated differential proteins was 17.99, and at least 90.77% of differential proteins were not randomly generated. This demonstrates that the differential proteins identified by the different time points contrast are not randomly generated. According to differential protein GO analysis and KEGG pathway analysis, a large number of biological processes and signaling pathways related to growth and development were enriched, which provided the basis for urine proteome to reflect the short-term growth and development of rats, provided a means for in-depth and meticulous study of growth and development, and also provided an interfering factor that needs attention for animal experiments using 6-8-week-old rats to construct models. The results of this study demonstrated that the urinary proteome could see the difference of urinary protein in rats aged 6-8 weeks only 3-6 days apart, which broadened the sensitivity boundary of urinary proteomics and showed the sensitive and precise characterization ability of urinary proteome to body changes.
Peer Review Status:Awaiting Review
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