分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Fluorescence spectroscopy was employed to investigate the interaction between fluorophore fluoresceinamine (FA) and bovine serum albumin (BSA) under physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by FA is a result of the formation of a BSA-FA complex. Fluorescence quenching constants were determined using the modified Stern-Volmer equation to provide a measure of the binding affinity between FA and BSA. The results of the thermodynamic parameters G, H, and S at different temperatures indicated that several kinds of interactions, except for the electrostatic interactions play cooperative roles in BSA-FA association. Furthermore, the conformation of BSA upon interaction with FA was also studied by synchrotron fluorescence spectroscopy.
分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Bio-nanosensors (Bio-NSs) have attracted much attention recently due to their unique properties. Among all of the bio-NSs, the intracellular proton sensor is significant for biomedicine studies and clinic diagnosis. Proton nanosensors (PNSs) with different pH sensitive ranges could satisfy different research requirements. Here we report a facile method to build a PNS with a neutral to basic pH sensitive range, in which the commercial pH indicator, fluoresceinamine (FA), was covalently coupled to the carboxylic-rich amphiphilic polymer (AP) coated gold nanoparticles (AuNPs).
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