Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBP alpha

Subjects: Biology >> Biophysics submitted time 2016-05-11

Xiao, Tengfei Liu, Lihui Sun, Yu Luo, Huaxia Chen, Runsheng Xiao, Tengfei Liu, Lihui Sun, Yu Luo, Huaxia Chen, Runsheng Xiao, Tengfei Li, Hongling Li, Tangping Wang, Shihua Zhao, Robert Chunhua Liu, Lihui Sun, Yu Luo, Huaxia Dalton, Stephen

Abstract： C/EBP alpha is a critical transcriptional regulator of adipogenesis. HowC/EBP alpha transcriptionis itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR(adipogenic differentiation induced noncoding RNA), transcribed from a position similar to 450 bp upstream of the C/EBP alpha gene, that orchestrates C/EBP alpha transcriptionin vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBP alpha. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBP alpha locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBP alpha in cis.

Peer Review Status:Awaiting Review

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