Specific binding of Clostridium perfringens enterotoxin fragment to Claudin-b and modulation of zebrafish epidermal barrier

分类： 生物学 >> 生物物理学 提交时间： 2016-05-11

Zhang, Jingjing Yang, Zhenguo Chen, Huapu Wang, Sijie Fan, Yiming Zhang, Jingjing Piontek, Anna Qin, Zhihai Piontek, Joerg Ni, Chen

摘要： Claudins (Cldn) are the major components of tight junctions (TJs) sealing the paracellular cleft in tissue barriers of various organs. Zebrafish Cldnb, the homolog of mammalian Cldn4, is expressed at epithelial cell-cell contacts and is important for regulating epidermal permeability. The bacterial toxin Clostridium perfringens enterotoxin (CPE) has been shown to bind to a subset of mammalian Cldns. In this study, we used the Cldn-binding C-terminal domain of CPE (194-319 amino acids, cCPE(194-319)) to investigate its functional role in modulating zebrafish larval epidermal barriers. In vitro analyses show that cCPE(194-319) removed Cldn4 from epithelial cells and disrupted the monolayer tightness, which could be rescued by the removal of cCPE(194-319). Incubation of zebrafish larvae with cCPE(194-319) removed Cldnb specifically from the epidermal cell membrane. Dye diffusion analysis with 4-kDa fluorescent dextran indicated that the permeability of the epidermal barrier increased due to cCPE(194-319) incubation. Electron microscopic investigation revealed reversible loss of TJ integrity by Cldnb removal. Collectively, these results suggest that cCPE(194-319) could be used as a Cldnb modulator to transiently open the epidermal barrier in zebrafish. In addition, zebrafish might be used as an invivo system to investigate the capability of cCPE to enhance drug delivery across tissue barriers.

同行评议状态:待评议

 点击量 1597  下载量 836  评论