Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis

分类： 生物学 >> 生物物理学 提交时间： 2016-05-11

Wang, Chun-Yan Zhou, Xue-Ping Hong, Jian Zhang, Chuan-Xi Zhang, Qin-Fen Gao, Yuan-Zhu Ji, Gang Huang, Xiao-Jun

摘要： Maize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most local resolution at approximately 4 angstrom. The C alpha backbone was built based on residues with bulky side chains. The resolved C-terminus of the capsid protein subunit and obvious openings at the 2-fold axis demonstrated the compactness of the asymmetric unit, which indicates an important role in the stability of MCMV. The Asp116 residue from each subunit around the 5-fold and 3-fold axes contributed to the negative charges in the centers of the pentamers and hexamers, which might serve as a solid barrier against the leakage of genomic RNA. Finally, the loops most exposed on the surface were analyzed and are proposed to be potential functional sites related to MCMV transmission. (C) 2015 Elsevier Inc. All rights reserved.

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