摘要: Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysin PlyV12. The results showed that the chimeric lysin Ply187N-V12C had not only lytic activity similar to Ply187N against staphylococcal strains but also extended its lytic activity to streptococci and enterococci, such as Streptococcusdysgalactiae, Streptococcus agalactiae, Streptococcus pyogenes, Enterococcus faecium and Enterococcus faecalis, which Ply187N could not lyse. Our work demonstrated that generating novel chimeric lysins with an extended lytic spectrum was feasible through fusing a catalytic domain with a cell-binding domain from lysins with lytic spectra across multiple genera.
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期刊:
MICROBIAL BIOTECHNOLOGY
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分类:
生物学
>>
生物物理学
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引用:
ChinaXiv:201605.01262
(或此版本
ChinaXiv:201605.01262V1)
DOI:10.12074/201605.01262V1
CSTR:32003.36.ChinaXiv.201605.01262.V1
- 推荐引用方式:
Dong, Qiuhua,Dong, Qiuhua,Wang, Jing,Yang, Hang,Wei, Cuihua,Yu, Junping,Zhang, Yun,Huang, Yanling,Wei, Hongping,Zhang, Xian-En.(2016).Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci.MICROBIAL BIOTECHNOLOGY.[ChinaXiv:201605.01262]
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