Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： To study the anti-inflammatory of chemical constituents from the rhizome of ethnomedicine Rosa roxbunghii. The chemical constituents of fresh R. roxbunghii rhizome were studied by phytochemical separation and purification technology, and their structures were identified through physicochemical properties and spectral data. The inflammatory model of RAW264.7 induced by lipopolysaccharide (LPS) in vitro was established. The cell viability was detected by MTT assay, the release of inflammatory factor NO in the cell culture medium was detected by Griess method and the anti-inflammatory activity of each compound was evaluated through the kit. The results were as follows: (1)Fifteen compounds were isolated and identified as kaji-ichigoside F1(1), rosamultin(2), euscaphicacid(3),β-D-glucopyranosyl-(2a→1b)-2a-O-β-L-arabinopyranosyl-(2b→1c)-2b-O-β-L-arabinopyranosyl-(2c→1d)-2c-O-β-L-arabinopyranosyl-(2d→1e)-2d-O-β-L-arabinopyranosyl-(2e→1f)-2e-O-β-L-arabinopyranoside(4),catechin(5), 3-O-methylellagic acid-4´-O-β-D-xylopyranoside(6), 3-O-methylellagic acid-4´-O-α-L- rhamnopyranoside (7), tormentic acid (8), betulinic acid (9), spinosic acid (10) , arjunic acid (11), β-Sitosterol (12), β-Daucosterol (13), α-tocopherol (14) and n-hexacosane (15). Compounds 4, 6 and 7 were isolated from R. roxbunghii for the first time. (2)The results of in vitro bioactivity showed that compounds 1-7 significantly inhibited LPS induced NO release from RAW264.7 in a dose-dependent manner; The IC50 were 25.07, 24.56, 17.65, 9.87, 16.67, 40.83 and 34.98 μmol·L-1, respectively (dexamethasone as positive control 22.46 μmol·L-1 ). Anti-inflammatory activity of compounds 1-7 were showed better, among which compounds 3, 4 and 5 were slightly better than dexamethasone. The results indicated that triterpenoids, ellagic acids, flavonoids and oligosaccharides from R. roxbunghii were the main effective components and verified its anti-inflammatory effect in folk application.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-08-03 Cooperative journals: 《广西植物》
Abstract：羊毛甾烷三萜是南烛属植物主要活性成分之一，为进一步研究该属植物功效物质基础，结合民族民间对小果南烛的应用实际，该文利用硅胶、MCI、Sephadex LH-20、半制备高效液相色谱等植物化学技术手段对该植物 95% 乙醇提取物进行分离、纯化，综合理化性质和波谱数据对其化合物进行鉴定。结果表明：从小果南烛的茎、叶提取物中分离得到 14 个三萜 及 其 皂 苷 ， 分 别 鉴 定 为 mollic acid 3-O-α-L-arabinopyranoside (1) 、 mollic acid3-O-β-D-glucopyranoside (2)、cycloart-3,7-dihydroxy-24-en-28-oic acid (3)、白桦脂酸 (4)、1β,3α, 11α-trihydroxy-urs-12-ene (5)、 oleanderolide (6)、(Z)-马斯里酸-3-O-对香豆酸酯 (7)、木栓酮 (8)、坡模酸 (9)、2α, 3α-二羟基乌苏-12-烯-28-酸 (10)、科罗索酸 (11)、齐墩果酸 (12)、熊果酸 (13)、委陵菜酸 (14)。其中化合物1-7 首次分离自南烛属植物，化合物8-11，14 为首次从小果南烛植物中分离得到。
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