Subjects: Biology >> Bioengineering submitted time 2018-01-11 Cooperative journals: 《中国生物工程杂志》
Abstract：为初步探讨Herpud1在肾脏发育中的生物学作用，过表达Herpud1载体及敲低Herpud1载体被构建。载体被转染进后肾间充质细胞，通过RT-PCR和蛋白免疫印迹检测了上皮间质的转换过程(EMT)的标志蛋白E-钙粘蛋白(E-cadherin)，波形蛋白(Vimentin)，Snail蛋白及内质网应力的标志蛋白葡萄糖调节蛋白78(GRP78），真核起始因子2α (eIF2α）的表达变化，通过EDU细胞增殖实验检测了Herpud1对细胞的增殖的作用，同时利用细胞划痕实验验证了细胞的迁移能力。结果显示，与空白对照组相比，在Herpud1过表达的实验组中，E-cadherin的表达在mRNA和蛋白水平均是降低的，而Snail和Vimentin的表达则是上升的，并伴随着细胞增殖活性的降低和细胞迁移能力增加，同时内质网应力相关蛋白GRP78和eIF2α是升高的。在Herpud1敲低的实验组，E-cadherin的表达在mRNA和蛋白水平均是升高的，而Snail和Vimentin的表达下降，细胞迁移能力是降低并且细胞增殖活性是升高的，同时内质网应力相关蛋白GRP78和eIF2α也是降低的。这些结果证明了Herpud1可以促进MK3细胞EMT过程，提高细胞的迁移能力，抑制细胞的增殖活性，其机制可能与细胞的内质网应力相关。
Subjects: Psychology >> Social Psychology submitted time 2023-03-27 Cooperative journals: 《心理学报》
Abstract： Humans are a social species that are constantly involved in complex relationships, reacting to the actions of others, and intentionally or unintentionally changing our own behavior. Personality traits reflect the behavioral pattern of an individual's response to the environment, which also includes social behavior. In addition, the brain is also an important factor when discussing social networks. The brain provides biological mechanisms for human behavior, while social networks provide external triggers for these behaviors. Linking personality traits and brain activity to social networks can help us better understand the structure of group relationships, improve our understanding of individual human beings, and help us better predict individual social behaviors and find the rules of information transmission in interpersonal relationships. From the perspective of a network, we collected nine social networks from 94 undergraduate students in the same grade according to their different social needs. We used the graph theory and resting-state functional magnetic resonance imaging to explore the influence of personality traits on social networks based on individual popularity and closeness between individuals and the relationship between the similarity of brain resting-state functional connectivity and social distance between individuals. Specifically, regression analysis was carried out, with personality traits as the independent variables and the different degrees of social networks as the dependent variables. Then, a correlation analysis was performed for the social distance and similarity of personality traits. Finally, the correlation between the similarity of the brain networks and social distance was calculated. The results showed that (1) individuals with high conscientiousness were more popular in social networks requiring "trust" traits, while individuals with high agreeableness were more popular in social networks requiring "fun" traits. These findings showed that in the same group, there are different social networks according to social needs, and the popularity of individuals in different social networks is not similar as it will be affected by the corresponding personality traits; (2) In the social networks requiring "shared interests & values, " personality similarity and social distance between individuals were significantly negatively correlated. Personality similarity promotes interpersonal communication between individuals, which may be realized through interpersonal attraction induced by the similarity of values and interests; (3) In the same social network, there is a significantly negative correlation between similarities in functional connections (FCs) and social distance among individuals, and these FCs are mainly concentrated in the fronto-parietal task control network and the dorsal attention network. The similarity of resting brain FCs among individuals may promote interpersonal communication, possibly due to the similarity of individuals in cognitive control and environmental processing bias, which increases the interpersonal attraction and shortening the social distance between individuals. The results revealed the influence of personality traits on the structure of different social networks, the relationship between personality trait similarity among individuals, and the similarity between resting brain networks and social distance, which has important implications for understanding the structure of social networks, the formation rules, and the information transmission rules among them. In addition, this study discussed the relationship between the similarity of resting-state FC and social distance, providing new evidence for studies on brain synchronization in interpersonal communication and brain imaging evidence for the study of the relationship between the similarity of personality traits and social distance.#social networks, personality traits, resting-state functional connectivity
Subjects: Psychology >> Social Psychology submitted time 2021-07-27
Abstract： " "
Peer Review Status:
Awaiting Review
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