Subjects: Biology >> Botany submitted time 2018-06-12 Cooperative journals: 《广西植物》
Abstract： 无菌的猕猴桃种子是猕猴桃胚乳培养、实生苗微嫁接等技术的基础材料，利用消毒剂灭菌是常用的无菌种子采集手段，应用最广泛的消毒剂为升汞（Mercuric chloride）和次氯酸钠（NaClO）。该文提出了一种新的无菌猕猴桃种子采集方法无菌搅拌法，为了探索其准确性和应用性，该研究比较了0.2 %升汞灭菌20min、10%次氯酸钠灭菌20min、无菌搅拌法3种方式采集无菌猕猴桃种子的效果，及对种子萌发和幼苗形成的影响。结果表明：无菌搅拌法、0.2 %升汞灭菌20 min是稳定有效的无菌猕猴桃采集方式，10 %次氯酸钠灭菌20 min的采集效果不稳定；在相同的时间内，无菌搅拌法的种子发芽率最高，为89.90%，但发芽势较低，均可正常成苗；10%次氯酸钠灭菌20min的发芽率次之，与无菌搅拌法的种子无显著差异，发芽势和成苗率最高，分别为47.47%和67.86%，且有打破猕猴桃种子休眠的作用，整体作用类似于赤霉素（GA3）浸种的效果；0.2%升汞灭菌20min对猕猴桃种子的萌发有抑制作用，各项指标均显著低于无菌搅拌法种子，且生长缓慢。此外，无菌搅拌法是物理处理，对种子、操作人员、环境均无害。该研究证实了无菌搅拌法的实用性和优势，发现了次氯酸钠在打破猕猴桃种子休眠方面的作用，为其它同类型果实的无菌种子采集提供了参考。
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-07-18 Cooperative journals: 《广西植物》
Abstract： Actinidia chinensis ‘Yangshi Jinhong 50’is a fine Actinidia chinensis Planch cultivar of yellow-flesh andred-heart,which favored bypeopleandknown as rich nourishment.In orderto establishtherapidandefficient propagation system in virtro, this study used stem with axillary bud of Actinidia chinensis ‘Yangshi Jinhong 50’as the explant, and used tissue culture to study suitable explants sterilization method, best plant growth regulator combination in Actinidia chinensis ‘Yangshi Jinhong 50’. This study founded two kinds of in vitro regeneration modes. Mode I : the axillary buds of stem with axillary bud were induced directly; Mode II : the callus was induced first, and then the adventitious buds were induced. The results showed that the explants best sterilization method was 75% alcohol 30 s+ 15% Ca(ClO)2 5 min+ 0.1% mercuric chloride 8 min; In Mode I, the plant growth substances combination of MS+ 4.0 mg·L-1 6-BA+ 0.1 mg·L-1 NAAfor axillary buds germination had the best the induction rate, which could reach 91.67%; In Mode II, callus rate of inducing stems bottom was more than 80%, the optimal medium that induced stems bottom callus to generate adventitious buds was MS+ 3.0 mg·L-1 6-BA+ 0.3 mg·L-1 NAA, germination rate reach 83.33%; the best plant growth substances combination in tufted bud culture was MS+ 4.0 mg·L-1 6-BA+ 0.4 mg·L-1 NAA, multiplication coefficient as high as 6.14; the best plant growth substances combination in rooting culture was 1/2 MS+ 0.9 mg·L-1 IBA, getting the differentiation of adventitious root about 15 d and complete plant 45 d, rooting rate reach 72.64%, average rooting number reach 25.67, average root length reach 2.67 cm, average plant height reach 3.08 cm. After the seedlings rooted, transplanted the seedlings to the substrate, theseedlings could reach the 96% survival rate in the substrate with the garden soil∶sand = 1∶1. Through this study, the micropropagation system in vitro of Actinidia chinensis ‘Yangshi Jinhong 50’ was established, which provides the basis for the research of genetic transformation in Actinidia chinensis Planch, at the same time, the establishment regeneration system provides a good technical supportforseedlingmicropropagationandfactoryinActinidiachinensis‘YangshiJinhong50’.
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