分类: 物理学 >> 核物理学 提交时间: 2016-09-13
摘要: Cross sections for the production of pairs of photons plus two additional jets produced from double parton scattering in high-energy proton-proton collisions at the LHC are calculated for the first time. The estimates are based on the theoretical perturbative QCD predictions for the productions of \gamma\gamma at next-to-next-to-leading-order, jet + jet and \gamma + jet at next-to-leading-order, for their corresponding single-scattering cross sections. The cross sections and expected event rates for \gamma\gamma + 2 jets from double parton scattering, after typical acceptance and selections, are given for proton-proton collisions with the center-of-mass energies of 13 TeV and an integrated luminosity of 100 fb-1 planned for the following two years, and also center-of-mass energies of 14 TeV with 3000 fb-1 of integrated luminosity as LHC designed.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: Tumor-resident proteases (TRPs) are regarded as informative biomarkers for staging cancer progression and evaluating therapeutic efficacy. Currently in the clinic, measurement of TRP is dependent on invasive biopsies, limiting their usefulness as monitoring tools. Here we identified circulating peptides naturally produced by TRPs, and evaluated their potential to monitor the efficacy of anti-tumor treatments. We established a mouse model for ovarian cancer development and treatment by orthotopic implantation of the human drug-resistant ovarian cancer cell line HeyA8-MDR, followed by porous silicon particle-or multistage vector (MSV) -enabled EphA2 siRNA therapy. Immunohistochemistry staining of tumor tissue revealed decreased expression of matrix metallopeptidase 9 (MMP-9) in mice exhibiting positive responses to MSV-EphA2 siRNA treatment. We demonstrated, via an ex vivo proteolysis assay, that C3f peptides can act as substrates of MMP-9, which cleaves C3f at L-1311-L-1312 into two peptides (SSATTFRL and LWENGNLLR). Importantly, we showed that these two C3f-derived fragments detected in serum were primarily generated by tumor-resident, but not blood-circulating, MMP-9. Our results suggested that the presence of the circulating fragments specially derived from the localized cleavage in tumor microenvironment can be used to evaluate therapeutic efficacy of anti-cancer treatment, assessed through a relatively noninvasive and user-friendly proteomics approach.