Subjects: Biology >> Botany submitted time 2022-10-27 Cooperative journals: 《广西植物》
Abstract： The beneficial microbiome assist the plant in disease defense. The root, stem and seed of Sophora tonkinensis from wild type were germinated and grew healthily, but they in cultivated type were susceptible to insect and pathogen attack. In order to explore the possibility of disease control using beneficial endophytic fungal microbiome harbored in S. tonkinensis from healthy wild type, endophytic fungi were isolated from the root, stem and seed of S. tonkinensis from healthy wild type, and then were further identified based on morphological characters and ITS sequence characters. Phylogenetic tree, α-diversity index and β-diversity index were used to analyze the phylogenetic evolution, diversity and similarity among different endophytic fungal microbiomes. The pathogen-resistant function in vitro of endophytic fungal microbiome was determined by the agar plug method and flat-stand method. The results were as follows：(1) 131 strains with 23 taxa, 108 strains with 23 taxa, and 64 strains with 11 taxa, were respectively isolated and identified from the root, stem and seed of S. tonkinensis; (2) more endemic genera and all endemic species, indicated that endophytic fungal microbiomes harbored in the root, stem and seed had tissue specificity in genus and species evolutionary tree; (3) The low β-diversity indicated that taxonomic similarity was very low among different endophytic fungal microbiomes, and consequently endophytic fungal microbiomes had tissue specificity in species evolutionary tree; (4) The high α -diversity showed that endophytic fungal microbiomes harbored in the root, stem and seeds, were abundant biodiversity; (5) More than one third of the taxa antagonized pathogens in vitro. Endophytic fungal microbiome harbored in the root/stem showed strong broad-spectrum pathogenic bacteria/fungi-resistant function in vitro, and endophytic fungal microbiome harbored in the seeds showed strong broad-spectrum pathogenic fungi-resistant function in vitro. These results indicated beneficial endophytic fungal microbiome with tissue-specificity and biodiversity structure, and strong broad-spectrum and abundant pathogen-resistant function in vitro, harbored in healthy root, stem and seeds of S. tonkinensis from wild type, and may play an important role in pathogen resistance in host tissues. Such results will provide material and experimental basis for disease control in different tissues of S. tonkinensis using beneficial endophytic fungal microbiome.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-02-14 Cooperative journals: 《广西植物》
Abstract： Eucalyptus L. Herit is a large genus of myrtidae with more than 600 species, native to Australia and some of its northern islands and mainly distributed in tropical and subtropical regions of the world. Eucalyptus has the characteristics of fast growth, high yield, short rotation period and so on. It is an excellent pulp material with remarkable economic benefits. There are many introduced species in China, mainly distributed in south China. Nearly 80 species have been introduced into China since 1890, widely distributed in Guangdong, Guangxi, Guizhou, Sichuan, Yunnan, Jiangxi and other places. At present, China has become one of the countries with the largest area of Eucalyptus plants in the world and is also the largest exporter of Eucalyptus oil. Eucalyptus has a lot of industrial value. Its wood, leaves and fruits are important raw materials in chemical industry, fragrance and medicine fields. They can be used for developing high performance Eucalyptus recombination material, bamboo Eucalyptus composite material, pulp and paper-making, etc. Eucalyptus plants are used as folk medicine, with anti-bacterial and anti--inflammatory, wind-relieving and anti-pyretic, anti-corrosion and anti-pruritic effects. As a traditional medicine, Australian aborigines were the first to use Eucalyptus for the treatment of fever and bronchial diseases; in China, the leaves of E. globulus Labill., E. robusta Smith., E. exserta F.Muell., E. tereticornis Smith., E. citriodora Hook. are often used to treat influenza, dysentery, eczema, and injuries for a long time. Pharmacological studies show that Eucalyptus plants have good anti-oxidant, anti-inflammatory, anti-bacterial, anti-viral, anti-tumor, anti-cardiovascular diseases and other pharmacological activities. In this study, 421 non-volatile chemical constituents from different parts of Eucalyptus and their pharmacological activities were classified and described in detail by referring to the domestic and foreign literatures related to Eucalyptus in recent 30 years. There were 73 flavonoids, 61 organic acids, 45 terpenoids, 229 polyphenols and 13 fatty alcohols. Most of the pharmacological activities are anti-oxidant, anti-bacterial, anti-viral and anti-tumor, but the related mechanisms still need to be further elucidated. The purpose of this study was to focus on the medicinal parts of Eucalyptus, fully explore their medicinal value, and carry out clinical transformation and new drug research, which could provide scientific basis for further research, development and utilization of Eucalyptus in the future.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： Corrected the error Latin name of the Zanthoxylum. nitidum var. nitidum type 3 by referring to the literatures and rearch. Determination of the five constituents in Z. nitidum by RP-HPLC, at the same time, detect if the Z. nitidum contains Toddaloactone and Compare with the Toddalia asiatica by RP-HPLC. The results showed that Toddaloactone is not included in Z. nitidum var. nitidum and Z. nitidum var. tomentosum. The content of active ingredients in different localities was significantly different. The total content of five active components in different types of Z. nitidum was analyzed by cluster, which could be divided into four groups, The result of cluster was consistent with types of Z. nitidum. This study clarifies the confusion between the Z. nitidum var. tomentosum and Z. nitidum var. nitidum type 3 in systematic botany, Avoid the use of the wrong Latin name by subsequent researchers. RP-HPLC method was used to analyze the difference of contents of 5 chemical components in different types and variety of Z. nitidum, which can provide important information for quality control of Z. nitidum and rational application and development of resources.
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