Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： The taxonomy of Tacca remains controversial. Schizocapsa guangxiensis is considered to be the same species as T. plantanginea, but some taxonomists classify them into different species based on their morphological differences. In order to clarify the genetic differences and phylogenetic relationship between S. guangxiensis and T. plantanginea, this study conducted high-throughput DNA sequencing of S. guangxiensis, assembled a complete chloroplast genome using bioinformatics software, and compared it with the published T. plantanginea chloroplast genome. The results showed that: (1) The chloroplast genome size of S. guangxiensis and T. plantanginea is 162 149 bp and 160 749 bp, respectively, and they have the same GC content (36.90%). Notably, the gene types and gene amount are exactly the same in these two species, including 89 proteincoding genes and 37 tRNA genes, and 6 rRNA genes. (2) Codon preference analysis shows that there are certain differences in the codon frequencies used by the two species, but they both prefer codons ending in A/T(U). (3) Compared with T. plantanginea, the SSC boundary of S. guangxiensis has obvious expansion, which is the main factor leading to the length variant in chloroplast genome between the two species. (4) There are some sequence divergences between S. guangxiensis and T. plantanginea in the LSC and SSC regions, especially the intergenic region, which can be exploited as species-specific molecular marker. (5) Phylogenetic results show that S. guangxiensis and T. plantanginea have a rather distant genetic relationship. Although S. guangxiensis is placed in Tacca, they belong to two different species. This study enriches the genetic information of the chloroplast genome of S. guangxiensis and provides a theoretical basis for species classification, genetic diversity analysis and species protection of S. guangxiensis.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： Vatica guangxiensis is a national first-class protected wild plant and extremely small populations wild plant. This paper provides a comparative analysis of the photosynthetic physiological characteristics and structural features of Vatica guangxiensis seedlings and adult trees. The Li-6400 portable photosynthesis measurement system analyzer and vacuum electron scanning microscope were utilized to investigate the differences between the photosynthetic capacity and leaf structure of leaves at different stages of growth and development. The results were as follows: (1) The right-angled hyperbolic modified model was the best model to fit the light response of Vatica guangxiensis seedlings and adult plants. (2) The maximum net photosynthetic rate(Pnmax), light saturation point (LSP) and dark respiration rate (Rd) of adult leaves were significantly higher than those of seedlings, the light compensation point (LCP) of adult plants was lower than that of seedlings(P<0.05). (3) Chlorophyll a, chlorophyll b, chlorophyll (a+b) and carotenoids in seedlings were significantly lower than in adult plants(P<0.05). (4) The stomatal long axis, stomatal organ area and stomatal density of adult plants were significantly higher than those of seedlings; the leaf area and specific leaf weight (SLW) of adult plants were significantly larger than seedling (P<0.05), and the specific leaf area (SLA) was smaller than seedling. In summary, Vatica guangxiensis exhibits photosynthetic characteristics of a shade-loving seedling and a sun-loving adult tree. The seedlings have weak light adaptation ability and light energy utilization, and are unable to photosynthesize sufficiently to meet their growth needs due to excessive shading by adult trees. This is a significant reason why seedlings are unable to grow into large trees. Therefore, for insitu conservation of Vatica guangxiensis, human interference can be used to artificially create ‘windows’ in the tree layer to provide sufficient light sources. This is conducive to the growth of Vatica guangxiensis seedlings into large trees and the renewal of the population. On the other hand, for the introductory cultivation of Vatica guangxiensis, the seedlings need to be appropriately shaded and transplanted to a certain degree of shade. During the initial cultivation process, it is important to provide appropriate shading for seedlings and transplant them to an environment with a suitable level of shade.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： Exploring the stoichiometric characteristics of understory plants’ leaves and soil in different forest types in Maoershan can reveal the adaptation strategies of understory plants in Maoershan, and provide data support for forest management. In this paper, coniferous and broad-leaved mixed forest (ZK), evergreen broad-leaved secondary forest (CLC) and evergreen broad-leaved forest (CL) in Maoershan were selected as three forest types, the leaf stoichiometry of main plants in herb layer and shrub layer, and the soil stoichiometry under three forest types were measured and analyzed. The results were as follows: (1) There was no significant difference in leaf C and N contents between herb layer and shrub layer, but P and K contents in herb layer were extremely significantly higher than that in shrub layer, and N∶P was significantly lower than that in shrub layer. Plants in herb layer was more likely to be restricted by N, plants in shrub layer was more likely to be restricted by P and the utilization efficiency of N and P were higher. There was no significant difference in leaf stoichiometry of plants in shrub layer among different forest types, but there were significant differences in leaf N content, C∶N, C∶P of plants in herb layer among different forest types. Plants in herb layer of coniferous and broad-leaved mixed forest had higher nutrient use efficiency. (2) The soil C and N contents of the three forest types showed that CL > CLC > ZK, and there were extremely significant differences among the three forest types. The soil P content of coniferous and broad-leaved mixed forest was the highest, while that of C∶P and N ∶ P was the lowest. (3) Soil in coniferous and broad-leaved mixed forest significantly affected some leaf stoichiometry of plants in herb layer and shrub layer, while the other two forest types had no significant effect on underforest plants. To sum up, there are significant or extremely significant differences in soil stoichiometry among different forest types in Maoershan. The nutrient requirements and environmental adaptation strategies of plants in herb layer and shrub layer are different. The soil of coniferous and broad-leaved mixed forest has a strong influence on the leaf stoichiometry of understory plants, and the soil with low decomposition efficiency of organic matter in this forest type, and the soil is limited by N due to the low decomposition efficiency of organic matter, so the management of N in the mixed forest should be strengthened.
Subjects: Biology >> Botany submitted time 2022-12-29 Cooperative journals: 《广西植物》
Abstract：
Gupo Mountain is adjacent to the Nanling Biodiversity Conservation Priority Area of China, which nurtures rich biological resources. To understand the diversity of macrofungal species in this area, a survey and collection of macrofungal resources in Guangxi Gupo Mountain Provincial Nature Reserve (abbreviate as Gupo Mountain) and adjacent areas was conducted by random tracking method, and combined morphological and molecular biological methods to identify. The results were as follows: (1) A total of 348 species macrofungal were found in this area, belonging to 175 genera in 69 families in 2 phyla, 6 orders and 17 classes, including 40 species of edible mushrooms, 42 species of medicinal mushrooms and 7 species of toxic mushrooms. (2) The analysis of the species composition of the region shows that the dominant families were nine families, including Agaricaceae, Boletaceae, Entolomataceae and Hymenochaetaceae, etc.; the dominant genera are eleven genera, including Amanita, Crepidotus, Crinipellis, Entoloma, etc. (3) There are two endemic genera in China, namely Fragiliporia and Zangia, and 11 endemic species, namely Coltricia crassa, Coltricia weii, Entoloma caespitosum, Entoloma praegracile, Entoloma subtenuicystidiatum, Fomitiporia tenuitubus, Fomitiporia torreyae, Fragiliporia fragilis, Perenniporia nanlingensis, Perenniporia subtephropora and Tremella cerebriformis. (4) Analysis of the funga composition of macrofungi in Gupo Mountain and adjacent areas revealed that the composition of families can be divided into cosmopolitan element (66.67%), pantropic element (17.39%) and north temperate element (15.94%); while the genera can be divided into seven elements, among which the cosmopolitan element is the most dominant (61.71%), and pantropic element (20.57%) and north temperate element (13.71%) rank second and thirdly. The rich species data provide basic information for the assessment of macrofungal species diversity and sustainable utilization of resources in Gupo Mountain and adjacent areas.
Subjects: Biology >> Botany submitted time 2022-09-29 Cooperative journals: 《广西植物》
Abstract： Corydalis saxicola is a perennial herbaceous plant, which mainly distributed in the karst areas of southwest China. Due to its important ecological and medicinal value, Corydalis saxicola has great prospects for development and utilization. With fragile living environmental condition and anthropogenic harvesting pressure, the wild resource of Corydalis saxicola is on the verge of exhaustion, so it has been included in the list of national key protected wild plants in 2021. In order to explore the causes for the endangerment of Corydalis saxicola and provide reference for its conservation and seedling breeding, this paper investigated the influencing factors on seed germination that including seed preservation methods, chemical pre-treatments, temperature, light, drought, pH, mix with wet sand and refrigerate treatment. The results were as follows: (1) Low-temperature storage can prolonged the seed activity, germination rate of 2-years stored seed could still reached 30%. (2) Germination rates of seed pre-treatments by HCl and NaClO solutions can reached to 50%~60%; germination rate was 50% at 20 ℃, while closed to zero at 30 ℃; darkness was more favorable for seed germination than periodic light condition; the seed germination rate kept decreasing with deepening drought; the seed germination rate did not changed significantly under the conditions of pH 3.0~8.0. (3) Mix with wet sand and refrigerate treatment can significantly promoted the seed germination potential and germination rate, and the germination rate was twofold of the control. In summary, the seed inactivation at room temperature and germination inability at high temperatures above 30 ℃ are more related to its endangerment; efficient seed germination methods can provide an effective way for conservation and industrial application of Corydalis saxicola.
Subjects: Biology >> Botany submitted time 2022-09-08 Cooperative journals: 《广西植物》
Abstract： Ardisia humilis is an excellent wild ornamental plant. For the purpose of fully utilization of this elite germplasm, the flowering biology and breeding system of this plant had been expre in this study. We conducted field investigation in the flowering phenology, floral morphology, and floral visitor on Ardisia humilis individuals introduced in homogeneous garden. Furthermore, pollen viability and stigma receptivity were detected by pollen germination in vitro and by benzidine-hydrogen peroxide method, respectively. Combined with artificial pollination,the self-compatibility and the self-pollination of Ardisia humilis were also analyzed in this study. The results were as follows: (1) The florescence of Ardisia humilis was from mid-late May to early June. The flowering span of the population, single plant and single flower were approximately 25 d, 8 d and 1 d, respectively. (2) Ardisia humilis developed protogynic bisexual flowers, with partial overlap on mature stage of pistil and that of stamen, while stigma remained receptivity until 1 d after stamens fall. (3) With a OCI value of 4 and pollen / ovule value of 5.61�103, the breeding system of Ardisia humilis were considered to be outcrossing, with partial self-compatibility, and the pollinator insects were essential. Lasioglossum sp.1, Lasioglossum sp.2 and Halictus sp. were the most observed floral visitors on Ardisia humilis.(4) In artificial pollination, The seed set of natural pollination ranked the highest level (averaged 52.96%), followed by that of autogamy (52.29%) and of artificial cross pollination (50.33%), while that of artificial geitonogamy (28.67%) was the lowest one. This study indicated that, beside of being excellent flowering plant, Ardisia humilis is also a potentially important ornamental fruit plant due to the generalized pollination system and the strongly autogamy ability which enhance the high success of fruits development from blossoms.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-05-21 Cooperative journals: 《广西植物》
Abstract： In order to study the chemical constituents in the leaves of Champereia manillana var. longistaminea, the chemical composition was analyzed by the silica gel column chromatography (CC), thin-layer chromatography (TLC), dextran gel column chromatography (Sephadex LH-20), reversed-phase silica (RP-18) gel column chromatography and high performance liquid chromatography (HPLC). The ethyl acetate extraction part of the ethanol extraction was separated and 6 monomer compounds were obtained. The structures of these compounds were identified using nuclear magnetic resonance spectroscopy (NMR), high resolution mass spectrometry (HR-ESI-MS) and the data of the literature. Six compounds were elucidated as Taraxerol (1), 1H-indole-3-carboxylic acid (2), (24R)-cycloartane-3β,24,25-triol (3), (24R,S)-3β-24,31-Epoxy-24-methylcycloartane(4),1-O-linolenoyl-3-O-β-D-galactospyranosyl-snglycerol (5), and Hyloglyceride (6). All compounds 1-6 were isolated from this plant for the first time.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Different varieties have different resistances. In order to further explore the resistance differences in different varieties of pitaya fruit and provide a reference for further study on breeding of pitaya fruit resistance. Our study using Illumina HiSeq 2000 sequencing platform to sequencing the transcriptome of ‘Putongbairou’ （BR） and ‘Ecuador Yellow’ （EY）. Functional classification and enrichment analysis of differentially expressed genes （DEGs） were performed by reference to GO Ontology, KEGG and others databases. The results were as follows: (1) There were 14 248 DEGs between BR and EY, of which 5 446 genes were up-regulated and 8 802 genes were down-regulated. (2) GO functional analysis showed that these DEGs are mainly involved in enzyme catalytic activity, cell components, metabolic processes, etc. Among them, there were 349 differential genes involved in oxidoreductase activity. (3) KEGG pathway analysis showed that most of the DEGs were enriched in metabolism, biosynthesis, etc. , and 12 key genes such as CYP86 and CER1 involving in Cutin, suberine and wax biosynthesis. We found the expression of DEGs involved in oxidoreductase activity were higher in BR than that in EY, which significantly enriched, indicating that may be differences in growth and cell metabolism between BR and EY. DEGs involved in the biosynthesis of Cutin, suberine and wax biosynthesis were up-regulated in BR. Such genes have higher expression in BR, and significantly enriched, suggesting that BR may be superior drought and disease resistance than EY.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-12-19 Cooperative journals: 《广西植物》
Abstract：锥栗C Castanea henryi Rehd. & Wils)是我国南方重要的木本粮食树种和特色果树资源，具有很高的食用及药用价值。为了探明最适宜锥栗种子萌发的条件，为锥栗种苗繁育提供理论基础，该试验以当年采收的锥栗种子为材料，研究了温度、光照、基质、基质含水量以及破壳处理等对锥栗种子萌发的影响。结果表明，锥栗种子萌发的最适温度是25℃，其最终萌发率为(92.672.67，在其它温度条件下，锥栗种子的萌发率有所下降，但与25 0C条件下的萌发率无显著差异;黑暗条件可缩短锥栗种子发芽时间，明显提高种子的萌发率和发芽势，但黑暗条件下的种子胚根长度显著小于周期性光照条件下的胚根长度;锥栗种子在泥炭土中的萌发率和发芽势均高于在河沙中的萌发率和发芽势，且泥炭土中种子胚根长度显著长于河沙中的种子胚根长度;基质含水量对锥栗种子萌发率和发芽势无显著性影响;种皮的机械阻碍是限制锥栗种子萌发的因素之一，破壳处理后，锥栗种子的萌发率明显提高，其发芽势和胚根长度与未破壳相比差异显著，实际生产中剪破种壳能加快种子的萌发进程。
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