Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-13 Cooperative journals: 《广西植物》
Abstract： To determine the genetic structure and sequence differences of the chloroplast genome of genus Aucuba and to reveal their interspecies relationship, the chloroplast genomes of six Aucuba species including A. chinensis and A. japonica var. variegata as well as Garrya buxifolia were sequenced using high-throughput sequencing technology. The chloroplast genomes were assembled and annotated using Bioinformatics software. The genome characteristics, sequence variation and phylogeny were analyzed based on the chloroplast genomes. The results were as follows: (1) Six Aucuba plants chloroplast genomes exhibited typical quadripartite circular structure (i.e., LSC, SSC, IRB and IRA) and were between 157 891-158 325 bp in length; (2) A total of 114 genes were annotated (except the duplicate genes on IRs), including 80 protein-coding genes (CDS), 30 tRNA genes and 4 rRNA genes; (3) 270 interspersed repeats, 133 tandem repeats and 412 SSR loci were detected in the six Aucuba chloroplast genomes; (4) A total of 29 high-frequency codons prefer ending in A/U. 100 optimal codons were filtered out in total, 12 of which were shared by six Aucuba species; (5) Comparative analysis of six Aucuba species reveals a highly conserved chloroplast genome sequence; (6) Ten highly divergent regions were identified based on nucleotide diversity analysis; (7) The phylogenetic trees were constructed using maximum likelihood (ML) and Bayesian (BI) showing a similar topology. The results support that genus Aucuba was monophyletic, as a sister to genus Garrya. We first sequenced the complete chloroplast genomes of these five Aucuba species and one Garrya species. The results reveal the interspecies relationships and the taxonomic status of genus Aucuba, and provide genetic resources for the classification, identification and phylogeny of the genus Aucuba.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-10-20 Cooperative journals: 《广西植物》
Abstract：为探求促进广金钱草萌发及生长的有效途径，该文使用不同浓度的费氏中华根瘤菌、放射型根瘤菌以及田菁茎瘤固氮根瘤菌菌液分别浸染广金钱草种子，以蒸馏水浸泡种子为空白处理，考察其对广金钱草种子萌发指标（发芽率、发芽势、发芽指数、活力指数）的影响，温室培养 40、60、80 d 后分别测定广金钱草幼苗的生长指标（叶片数、分枝数、株高）及部分生理指标（叶绿素含量、含氮量）的变化。结果表明：在 1107 CFU‧mL-1 浓度条件下，三种菌液浸种均获得最大萌发指标，其中田菁茎瘤固氮根瘤菌实验组效果最显著，发芽率、发芽势、发芽指数和活力指数高于对照组 16.00%、9.33%、9.51 和 41.34%；除田菁茎瘤固氮根瘤菌实验组外其余实验组的广金钱草幼苗叶片数、分枝数及株高均低于对照组；放射型根瘤菌实验组和田菁茎瘤固氮根瘤菌实验组幼苗叶绿素含量相比对照组分别增加 1.47%和7.47%，含氮量分别增加了 0.57%和 5.17%。综上所述，三种根瘤菌菌液浸种均可在不同程度上提高广金钱草种子的发芽能力和植株生长期的叶绿素和含氮量，其中田菁茎瘤固氮根瘤菌具有最大正向影响，可有效提高种子的萌发能力和促进植株生长。本研究可为不同地区广金钱草的种植和栽培提供技术指导，为开发田菁茎瘤固氮根瘤菌作为植物促生长菌剂提供理论支持。
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