Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-26 Cooperative journals: 《广西植物》
ZHENG Huachan
MENG Lingru
HE Miao
HUANG Wenli
DAN Linwei
XU Hong
DENG Chong
ZHANG Huawei
JIANG Yi
SONG Xiaomei
Abstract： The aerial part of Panax japonicus was called “Zhuzishen Ye”, it is a characteristic Chinese herbal medicine in Qinba area. In order to rationally utilize “Zhuzishen Ye” and clarify its chemical constituents, the main chemical constituents of the saponins fraction from “Zhuzishen Ye” were analyzed with HPLC, the inhibitory activity and inhibition type of the saponins fraction of “Zhuzishen Ye” on lipase were determined, and the lipase inhibition mechanism and hypolipidemic effect were verified with molecular docking and animal experiments. The results were as follows: (1) The chemical constituents of saponins fraction from “Zhuzishen Ye” were 20(S)-Ginsenoside Rg2, 20(R)-Ginsenoside Rg2, Ginsenoside Rb2, Ginsenoside Rb3, Ginsenoside Rd and Ginsenoside Rh2. (2) The saponins fraction of “Zhuzishen Ye” and 20(R)-Ginsenoside Rg2 had strong inhibitory effect on lipase with IC50 of 0.14 and 2.30 μmol·L-1 respectively. (3) The saponins fraction of Zhuzishen Ye, 20(R)-Ginsenoside Rg2 and Ginsenoside Rb3 were all reversible inhibition, and the inhibition type was non-competitive inhibition. (4) The binding of ligands to ARG337B, ASP331B and ILE248B residues may help to improve the lipase inhibitory activity of ligands. (5) Saponins of “Zhuzishen Ye” could significantly reduce the content of cholesterol and triglyceride in serum of hyperlipidemia mice. This study laid a foundation for the further development and utilization of “Zhuzishen Ye”.
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