Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2021-03-09
Abstract： Objective To explore the possibility of establishing a rabbit model of common carotid artery-external jugular vein arterivenous fistula with modified no-touch technique. Methods 10 male New Zealand rabbits were randomly divided into experimental group and sham operation group.Common carotid artery-external jugular vein arterivenous fistulas were created by the modified no-touch technique in experimental group, which means the perivenous vascular tissue was not stripped off in the operation.And the vessels were not anastomosed in sham operation group. At 14 days after surgery, We used Doppler Ultrasound to evaluate the changes of arterivenous fistula vascular inner diameter and blood-flow spectrum.We used hematoxylin-eosin (H-E) staining to observe the changes of intima thickness. And we took the blood samples from the rabbit’s external jugular vein for blood gas experiment. Results In experimental group, all fistulas were successfully established.100% success rate.14 days after surgery,Doppler ultrasound showed that external jugular vein was significantly expanded(P=0.016) and peak systolic velocity(PSV) was significantly increased in experimental group （P=0.015）. Compared with sham operation group, ultrasonographic examination of the common carotid artery and external jugular vein at 14 days after surgery showed obvious dilation of inner diameter and significantly increased of peak systolic velocity(PSV) in experimental group. The blood-flow spectrum of the common carotid artery and external jugular vein showed spiral laminar flow. H-E staining examination showed that elastic membrane of external jugular vein was destroyed and intima thickness was significantly increased in experimental group(P<0.001). Moreover, the PO2 in the blood of the external jugular vein in experimental group was significantly higher（P=0.006）.Conclusion The modified no-touch technique can successfully establish rabbit common carotid artery-external jugular vein arterivenous [Abstract]Objective To explore the possibility of establishing a rabbit model of common carotid artery-external jugular vein arterivenous fistula with modified no-touch technique. Methods 10 male New Zealand rabbits were randomly divided into experimental group and sham operation group.Common carotid artery-external jugular vein arterivenous fistulas were created by the modified no-touch technique in experimental group, which means the perivenous vascular tissue was not stripped off in the operation.And the vessels were not anastomosed in sham operation group. At 14 days after surgery, We used Doppler Ultrasound to evaluate the changes of arterivenous fistula vascular inner diameter and blood-flow spectrum.We used hematoxylin-eosin (H-E) staining to observe the changes of intima thickness. And we took the blood samples from the rabbit’s external jugular vein for blood gas experiment. Results In experimental group, all fistulas were successfully established.100% success rate.14 days after surgery,Doppler ultrasound showed that external jugular vein was significantly expanded(P=0.016) and peak systolic velocity(PSV) was significantly increased in experimental group （P=0.015）. Compared with sham operation group, ultrasonographic examination of the common carotid artery and external jugular vein at 14 days after surgery showed obvious dilation of inner diameter and significantly increased of peak systolic velocity(PSV) in experimental group. The blood-flow spectrum of the common carotid artery and external jugular vein showed spiral laminar flow. H-E staining examination showed that elastic membrane of external jugular vein was destroyed and intima thickness was significantly increased in experimental group(P<0.001). Moreover, the PO2 in the blood of the external jugular vein in experimental group was significantly higher（P=0.006）.Conclusion The modified no-touch technique can successfully establish rabbit common carotid artery-external jugular vein arterivenous fistula.This model has an ideal success rate and good repeatability, which provides an ideal animal model for further research on the application of modified no-touch technique in arteriovenous fistula surgery.fistula.This model has an ideal success rate and good repeatability, which provides an ideal animal model for further research on the application of modified no-touch technique in arteriovenous fistula surgery. " " "
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Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2021-03-08
Abstract：
Peer Review Status:Awaiting Review
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