Subjects: Biology >> Botany >> Applied botany submitted time 2023-09-20 Cooperative journals: 《广西植物》
Abstract： In order to identify the pathogens that caused tuber rot in Bletilla striata and study the inhibiting effects of herbal extracts on pathogens, the pathogens that caused tuber rot of Bletilla striata were isolated using usual tissue isolation. Morphological and molecular biological techniques were used to identify the strains. And seven herbal extracts were used to study the inhibiting effects on the pathogen. The results were as follows:（1） A total of 14 fungi and 4 bacteria were isolated from diseased leaves, leaf sheaths and tubers. But only strain GF-1 caused disease, whose symptoms consistent with those in the field. The incidences of GF-1 disease reinoculated in the field and laboratory were 100％, respectively. （2）GF-1 was identified as a memmber of Epicoccum, and its colonial morphology is a circular form, with white mycelium, prostrate on the medium, aerial, diaphragms and branches. There are conidia and chlamydospores. （3）At last, the sequence of internal transcribed spacer (ITS) region of GF-1 were analyzed, the length was 522 bp. The sequence was compared with other species in the GenBank and reached 99.62% similarity to E. sorghinum (MN493119.1) isolated form Sorghum, which was closer than others, including E. sorghinum (MF948994.1) isolated form leaves of B. striata. （4）GF-1 could be fully inhibited when the medium contained 0.1- 0.2 g·mL-1 extracts that extracted from 7 Chinese herbal, respectively. And it also could be fully inhibited by 0.05 g·mL-1 of C. cassia or Syringa oblate. In summary, the pathogen that caused tuber rot in Bletilla striata was identified as E. sorghinum. And GF-1 could be fully inhibited cultivated on the medium which contained 0.1- 0.2 g·mL-1 herbal extracts, e.g.: C. cassia, S. oblate, Cyclocarya paliurus, B. striata, Houpoea officinalis, Illicium verum or Cnidium monnieri.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》
Abstract： Inorder to study the effect of soil factors on the the growth and medicinal component of Paris polyphylla var. chinensis, and to provide a support for the artificial cultivation, the biomass and the polyphyllins content of different samples from different origins were determined, and soil composition was measured. Then the correlation between soil factors and the growth ， and medicinal component were analyzed. The results were as follows: (1)There were differences in soil composition in different place, while the yield and polyphyllins content in samples in different place were quite different; (2)Correlation analysis showed that the dry weight was significantly positively correlated with organic matter, total nitrogen, and alkaline nitrogen, the polyphyllin I content was significantly positively related with organic matter and available phosphorus, the polyphyllin Ⅱ content was significantly positively correlated with available phosphorus and available potassium; (3)Multiple linear regression analysis showed that the dry weight was dominantly affected by alkaline nitrogen, the polyphyllin I was dominantly affected by organic matter, the polyphyllins Ⅱ was dominantly affected by available phosphorus, and was linear negatively correlated with alkaline nitrogen. In summary, the main soil factors affecting dry weight was alkaline nitrogen, the main soil factors affecting the polyphyllin total content was organic matter and available phosphorus.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-13 Cooperative journals: 《广西植物》
Abstract： In order to establish a rapid propagation system of southern highbush blueberry ‘O’ Neal’ , the stem section with axillary bud were sterilized as explants, induction of shoot cluster,and proliferation of shoot cluster, test-tube rooting, transplanting and domestication were also studied. The results were as follows: The best sterilization treatment was that the explant treated by 10% NaClO with 15 min, and the contamination rate reached 14.44% and the induction rate reached 49.38%; The best medium for induction of shoot cluster was WPM+ ZT 1.5 mg‧L-1. The optimum medium for proliferation of shoot cluster was WPM+ NAA 0.1 mg‧L-1 + ZT 1.5 mg‧L-1, and the coefficient of propagation reached 8.6 after subcultured 60 days. The best treatment for test-tube rooting was that the shoot cluster dipped in 500 mg‧L-1 IBA for 20 s firstly, then cultivated them with the medium WPM+IBA 0.2 mg‧L-1, the rooting rate and effective rooting rate all reached 96.3%, and the rooting number and length were 12.7 and 43.3 mm after cultured 90 days, at the same time, and the root developed well. The survival rate of rooted seedlings reached 92.22% after transplanted in Media Ⅰ（river sand﹕vermiculite﹕perlite=1﹕1﹕1）. Thus, the best rapid propagation system of southern highbush blueberry ‘O’Neal’ was that: the explant treated by 10% NaClO with 15 min, the shoot cluster inducted and proliferated with WPM+ ZT 1.5 mg‧L-1 and WPM+ NAA 0.1 mg‧L-1 + ZT 1.5 mg‧L-1differently; The shoot cluster dipped in 500 mg‧L-1 IBA for 20 s and cultivated with WPM+ IBA 0.2 mg‧L-1, and rooted seedlings transplanted in media（river sand: vermiculite: perlite=1﹕1﹕1）. The results play a foundation for providing seedlings and factory production of ‘O’Neal’ in the future.
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