Subjects: Biology >> Botany >> Applied botany submitted time 2020-05-28 Cooperative journals: 《广西植物》
Abstract： In this study, the protein members of AP2 gene family in Auxenochlorella protothecoides are predicted and analyzed in detail by bioinformatics, which provided some ref-erence for further study on the function of AP2 transcription factor in Auxenochlorella protothecoides. Eight protein members of the AP2 family of Auxenochlorella protothecoides are analyzed by online tools such as PrtoParam, Pfam 3.20 and Protscale and so on. The results show that amino acids numbers of the family members are 247(XP_011395646.1) to 715(XP_011401904.1). The maximum theoretical isoelectric point is 9.39(XP_011396011.1), and the minimum is 5.81(XP_011398158.1). The positions and numbers of conserved domains in each member of the family are different; all protein members have no signal peptide; do not con-tain transmembrane helix, do not have transmembrane region; The maximum hydrophilicity value of the protein members is -3.222(XP_011398158.1), and the maximum hydrophobicity value is 2.333(XP_011401904.1), and the average hydrophilicity/hydrophobicity value of all members is less than 0; The values of many phosphorylation sites in the members were far beyond the stand-ard value of 0.5, and the maximum value of phosphorylation site is 0.998. The component content of secondary structures of protein members in the gene family is arranged in the order of ran-dom coil > alpha-helix > extended strand > beta-turn. It is speculated that alpha-helix and random coil are the main modes of its secondary structure, while extended strand and beta-turn are dis-persed in the amino acid chain of all protein members.Alpha-helix, beta-sheet, beta-turn, N-terminal and C-terminal were observed in the tertiary structure of all members. Phylogenetic analysis showed that Ostreococcus tauri is the most distant relative to the Auxenochlorella protothecoides, The closest relatives are Chlorella variabilis NC64A and Helicosporidium, and the next closest relatives are Picochlorum sp. SENEW3 among the other 15 species.This study systematically analyzed the physicochemical properties, conserved motifs and phylogenetic rela-tionships of AP2 protein family in Auxenochlorella protothecoides. It provided a reference for further study on the function and interaction of AP2 transcription factors in Auxenochlorella protothecoides.
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