Subjects: Biology >> Molecular Biology submitted time 2023-05-05
Abstract： Urine proteome can reflect how short-term changes in growth and development of animal organisms and whether short-term developmental effects on urinary protein need to be considered when performing urine marker studies using animal models built with faster growing periods? In this study, urine samples were collected from 10 Wistar rats aged 6-8 weeks 3 and 6 days apart and analyzed using a non-labeled quantitative proteomics technique with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The results showed that urine proteome could sensitively reflect the changes of short-term growth and development in rats. For example, comparing the urine proteome of Day0 and Day6, 195 differential proteins could be identified after screening (FC ≥ 1.5 or ≤ 0.67, P < 0.05), and verified by randomization, the average number of randomly generated differential proteins was 17.99, and at least 90.77% of differential proteins were not randomly generated. This demonstrates that the differential proteins identified by the different time points contrast are not randomly generated. According to differential protein GO analysis and KEGG pathway analysis, a large number of biological processes and signaling pathways related to growth and development were enriched, which provided the basis for urine proteome to reflect the short-term growth and development of rats, provided a means for in-depth and meticulous study of growth and development, and also provided an interfering factor that needs attention for animal experiments using 6-8-week-old rats to construct models. The results of this study demonstrated that the urinary proteome could see the difference of urinary protein in rats aged 6-8 weeks only 3-6 days apart, which broadened the sensitivity boundary of urinary proteomics and showed the sensitive and precise characterization ability of urinary proteome to body changes.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Pharmacology submitted time 2022-03-09
Abstract：
[Objective] The drug consistency evaluation of atorvastatin by urine proteomic analysis.
[Methods] Intragastric administration rat models were established by atorvastatin from two companies. Urine samples were collected from rats before and after intragastric administration. Urine proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and the biological pathway of the differential proteins was analyzed by DAVID database.
[Results] The differential proteins were selected by comparing the after intragastric administration to before intragastric administration. A total of 116 differential proteins were identified in group A and 66 differential proteins in group B, and 24 proteins were identified in common between two groups. These differential proteins tend to be involved in biological pathways such as cell adhesion, negative regulation of endopeptidase activity and proteolysis.
[Conclusions] For two kind of atorvastatin, we can detect small differences in urinary protein between them, which confirms the sensitivity of urinary protein and suggests that urine proteomics has great potential for the Drug consistency evaluation.
Peer Review Status:Awaiting Review
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