Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-26 Cooperative journals: 《广西植物》
Abstract： In order to understand the difference in microbial diversity between wild and cultivated Anemone altaica rhizosphere soil. and Illumina MiSeq high-throughput sequencing was used to study the microbial composition and diversity in rhizosphere soils between wild and cultivated A. altaica. The results were as follows: (1) Alpha diversity analysis showed that the diversity of fungi in the rhizosphere soil of wild A. altaica was significantly higher than that of cultivated A. altaica (P < 0.05), while the difference in bacterial diversity was not significant (P > 0.05). The non-metric multidimensional scaling (NMDS) revealed that the fungal community structures of wild and cultivated A. altaica rhizosphere soil had more significant differences. (2) A total of 9 566 operational taxonomic units (Otus) of bacteria belonged to 39 phyla, 127 classes, 315 orders, 500 families and 886 genera; and 2 670 operational taxonomic units (Otus) of fungi belonged to 15 phyla, 57 classes, 138 orders, 293 families and 597 genera. The bacterial phylum Proteobacteria, Acidobacteria, Actinobacteria and the fungal phylum Basidiomycota, Ascomycota, Mortierellomycota were the dominant phylas in the rhizosphere soil between wild and cultivated plants, despite their relative abundance was different. At genus level, Candidatus_udaeobacter, Norank_f_xanthobacteraceae and Rokubacteriales were the dominant rhizosphere soil bacteria of wild A. altaica. However, Candidatus_udaeobacter, Vicinamibacter and Rokubacteriales were the dominant rhizosphere soil bacteria of cultivated A. altaica. The dominant rhizosphere fungi of wild A. altaica were Mortierella, Sebacina and Russula, while the dominant rhizosphere fungi of the cultivated A. altaica were Sebacina, Mortierella and Unclassified_f_hyaloscyphaceae. (3) The redundancy analysis (RDA) showed that organic matter was the main factor affecting soil bacterial community (P < 0.05), and soil pH, alkali-hydrolyzable nitrogen and available phosphorus were the main factors affecting fungi communities (P < 0.05)., while bacterial community structure were not significantly correlated with soil pH, alkali-hydrolyzable nitrogen and available phosphorus (P > 0.05). In summary, our study revealed significant difference in the composition and diversity of rhizosphere microbial communities between wild and cultivated A. altaica, and the main soil factors influencing its rhizosphere microbial communities, the results of this study have certain significance for scientific planting and soil improvement of A. altaica.
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