Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-27 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of small interfering RNA (siRNA)-mediated silencing of PC4 and SFRS1 interacting protein 1 (PSIP1) on invasion and migration of human glioma U87 cells. Methods Chemically synthesized siRNA targeting PSIP1 gene was transfected into U87 cells via lipofectamine, and the gene silencing effect was determined using real-time PCR. The changes in the invasion and migration abilities of the transfected cells were assessed with Transwell assay and wound healing assay, respectively. Western blotting was used to analyze the expression of N-cadherin, β-catenin and the transcription factor Slug. Results The mRNA and protein level of PSIP1 was significantly reduced in U87 cells after transfection with PSIP1 siRNA (P<0.0001). PSIP1 knockdown in U87 cells resulted in significant suppression of cell invasion and migration abilities (P<0.01) and also reduced N-cadherin, β-catenin and Slug expressions. Conclusions Silencing of PSIP1 impairs the invasion and migration abilities of glioma cells and lowers the expressions of N-cadherin, β-catenin and Slug, suggesting that PSIP1 may regulate Slug by classical Wnt/β-catenin signaling pathway to modulate epithelial-mesenchymal transition and promote the invasion and migration of glioma cells.
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究大豆黄酮、芒柄花素及其组合对奶牛产奶性能、血浆和乳中激素含量的影响。选取健康年龄、泌乳期相近的40头荷斯坦奶牛，随机分为4组，分别为对照、Ⅰ、Ⅱ、Ⅲ组，每组10头。所有奶牛饲喂全混合日粮，试验Ⅰ、Ⅱ、Ⅲ组在此基础上分别添加2.50 g大豆黄酮、35.0 g芒柄花素、1.25 g大豆黄酮+17.5 g芒柄花素。进行127 d的饲养试验，其中预试期7 d，正试期120 d。结果显示：试验Ⅰ、Ⅱ、Ⅲ组产奶量与对照组相比差异显著（P0.05）；试验组的乳蛋白率和乳蛋白产量均高于对照组，其中试验Ⅲ组与对照组相比差异显著（P0.05）；试验组血浆和乳中雌二醇含量均高于对照组，试验Ⅲ组与对照组差异显著（P0.05）；各试验组血浆中催乳素含量均高于对照组，其中试验Ⅱ组与对照组差异显著（P<0.05）；试验组乳中雌马酚含量显著高于对照组（P<0.05）。因此，补饲大豆黄酮、芒柄花素及其组合可提高奶牛产奶量、改善乳品质，显著增加乳中雌马酚含量，同时使奶牛血浆和乳中雌二酮、孕酮含量增加。
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