Subjects: Biology >> Biochemistry submitted time 2022-02-28
Abstract：
Hypertension is a multifactorial complex disease related to genetic and environmental factors, which has become the most serious public health problem. Despite progress in antihypertension, there are limitations in the diagnosis and treatment of hypertension due to the lack of relevant molecular mechanisms. Urinary proteome has the potential to provide new research strategies for complex diseases involving multiple biological pathways and organs. In this study, the stroke-prone spontaneously hypertensive rats (SHRSP) were used. Urine samples were collected at months 1, 4, 8, 10, 12, and 14 during hypertension development. Dynamic urine proteomes were analyzed by using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Considering that the degree of disease progression may be different, each rat was compared before and after to screen for differential proteins, and functional annotation analysis was performed based on the differential proteins to have a better understanding of the pathological mechanisms involved in hypertension progression. During hypertension development, urine proteins were enriched some important pathways, such as renin-angiotensin signaling response to oxidative stress, aldosterone signaling in epithelial cells, renin-angiotensin signaling, IL-12 signaling and production in macrophages, apelin adipocyte signaling pathway, calcium transport I, STAT3 pathway, and glucocorticoid receptor signaling. These pathways were reported to be involved in the pathological mechanisms of hypertension, and might provide new clues for antihypertension drug target research. This study demonstrates that urinary proteomics can reflect the pathological processes associated with hypertension. This suggests that urine proteomic approaches may be used to investigate the mechanisms associated with hypertension and to find new drug targets of hypertension, and has the potential to provide personalized antihypertensive strategies with optimal efficacy.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2021-04-08
Abstract： "
Peer Review Status:Awaiting Review
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