Subjects: Biology >> Botany >> Plant biochemistry, plant Biophysics submitted time 2016-05-03
Maria Elisa Sabatini
Mattia Donà
Paola Leonetti
Andrea Minio
Massimo Delledonne
Daniela Carbonera
Massimo Confalonieri
Giorgio Giraffa and Alma Balestrazzi
Abstract：
The role of plant tyrosyl-DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α-depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.
Peer Review Status:
Awaiting Review
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