Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
XU Pengn
TAN Qiujinn
SONG Haiyunn
ZHENG Shufangn
YANG Xiaozhoun
HUAN Xiujun
ZHANG Taon
ZHOU Chunhengn
WEI Yuanrongn
WANG Wenlinn
Abstract： Macadamia nut (Macadamia integrifolia) is an evergreen nut tree with high economic value. Its kernel is rich in nutrients such as fatty acid and protein, etc. In order to further explore the main regulatory genes related to nutrient formation in M. integrifolia kernels, transcriptomics, gene cloning, fluorescence quantification PCR and bioinformatics techniques were used to screen potential regulatory genes from the kernel transcriptomes of ‘Guire No. 1’ and ‘A4’, which have significantly different nutrient content in M. integrifolia kernels. The results were as follows: (1) Transcriptome analysis showed that 1 667 genes were up-regulated and 1 798 genes down-regulated in ‘Guire No. 1’ kernel compared with those of ‘A4’ kernel and KEGG enrichment analysis showed that the differential genes were mainly in starch and glucose metabolism, amino acid biosynthesis and carbon metabolism. (2) A significant differential gene-LOC122077931 encoding the R2R3-MYB transcription factor MYB44L was discovered. The MiMYB44L gene was cloned in kernels of M. integrifolia variety ‘Guire No. 1’ using RACE technology, which was 1 165 bp in length, 999 bp in ORF in length, and encoded 332 amino acids. (3) Bioinformatics analysis confirmed the presence of the SANT domain in the MiMYB44L protein, a hallmark feature of the R2R3-MYB family. The protein lacked both a signal peptide and a transmembrane domain but featured phosphorylation sites. (4) The protein content in kernels of 10 M. integrifolia varieties was determined. And it was found that the expression of MiMYB44L gene in M. integrifolia varieties with high protein content was significantly higher than that in varieties with low protein content, and the overall correlation coefficient was 0.54, reaching a very significant level. The results of this study provide theoretical guidance for in-depth analysis of the regulatory mechanism of MiMYB44L gene in the formation of protein content in M. integrifolia.
Hits
Hits
Downloads
Comment