笔竹大、小孢子的发生及雌、雄配子体发育研究

Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-27 Cooperative journals: 《广西植物》

赵婉琪 国春策 王燕 肖姣 吴正春 刘海文 杨光耀 于芬

Abstract： To reveal the causes of low seed-setting rate of Pseudosasa viridula, the process of megasporogenesis and microsporogenesis and the development of female and male gametophyte of P. viridula were studied using traditional paraffin section and microscopy techniques. The results were as follows: Each flower contained three anthers (rarely 6) and an anther with four pollen sacs. The anther wall belonged to the basic type with the typical glandular tapetum, which composed of four layers of cells, which were epidermis, endothecium, middle layer and tapetum from outside to inside. The cytokinesis was the successive forming the medianly zygomorphic tetrads. Mature pollen grains were 2-cell type or 3-cell type, and some abortions occured. Mature pollen grains were 2-cell type or 3-cell type, and some abortions had occurred, which mostly occurred in the mononuclear stage of microspores. Its ovary was unilocular, with parietal placenta was anatropous, double integument and crassinucellate. The embryo sac was the polygonum type composed of an egg organ, a polar nucleus and three antipodal cells. The pistil structure was complete with embryo sac normal development, and the microspore abortion occurs in the single nuclear pollen stage. It is the typical male sterility and pollen abortion may be the main reasons for the low seed-setting rate of P. viridula. This study clarified the process of megaspogenesis, microsporogenesis and the development of female and male gametophyte of P. viridula, and initially revealed the reasons for the low seed setting rate of this species, which provides basic information for the development of cross breeding of P. viridula.

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