The effect and application of pCO2 in mammalian cell culture process

Subjects: Biology >> Bioengineering Subjects: Engineering and technical science >> Bioengineering submitted time 2018-12-11

刘小双 徐寒梅

Abstract： Carbon dioxide partial pressure (pCO2) is an important parameter in mammalian cell culture, which come from cell respiration, bicarbonate in culture media and CO2 added during culture for pH maintenance. Generally, pCO2 is not precisely controlled like pH in cell culture, but it is crucial to maintain pCO2 level within a reasonable range, usually ranging from 30 to 80 mmHg. Higher (> 80mmHg) or lower (< 30mmHg) pCO2 levels were reported to be detrimental to cell growth, metabolism, productivity and product quality. Higher pCO2 is always encountered during scale-up, especially for the high density perfusion culture. Therefore, pCO2 difference between small scale (< 15 L) and large scale (> 200L) needs to be considered. This review illustrates the effects of pCO2 on cell growth, metabolism, productivity and product quality. In addition, the approaches to control pCO2 during scale-up are also discussed. " "

Peer Review Status:Awaiting Review

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