Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-06-15 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate whether pigment epithelium-derived factor (PEDF) inhibits invasion and metastasis of breast cancer through regulation of epithelial-mesenchymal transition. Methods The expressions of PEDF, vimentin, and E-cadherin were detected in 119 breast cancer tissues using immunohistochemistry. SK-BR-3 breast cancer cell models of PEDF knockdown and PEDF overexpression were established by transfecting the cells with a PEDF-siRNA vector and a lentivirus-PEDF vector, respectively. Western blotting was used to detect the changes in the expressions of PEDF, vimentin, and E-cadherin in the cells, and the cell invasion and migration ability was assessed using scratch wound healing assay and transwell migration assay. Results PEDF positivity rate was significantly lowered in breast cancer tissues compared with the adjacent tissues. PEDF was positively correlated with the tumor size and the expression level of E-cadherin (r=0.473, P<0.001), but was negatively correlated with vimentin expression (r=-0.412, P<0.001). Transwell invasion experiment showed that PEDF interference enhanced the cell invasion and metastasis, while PEDF overexpression inhibited the invasion and migration of SKBR-3 cells. Western blotting showed that PEDF knockdown significantly decreased the expression of E-cadherin (P<0.05) and increased vimentin expression in the cells (P<0.05). Conclusion PEDF is closely related with the metastasis of breast cancer cells through regulation of epithelial-mesenchymal transition.
Subjects: Geosciences >> Atmospheric Sciences submitted time 2023-02-27 Cooperative journals: 《干旱区地理》
Abstract： Solar radiation is the primary energy source of the earth’s system and is closely related to human life. By selecting the monthly sunshine hour data of 50 meteorological observation stations in the Qinghai Plateau of China from 1961 to 2020, the spatiotemporal variation characteristics of sunshine hours in the Qinghai Plateau as a whole, different latitudes, and different altitudes were analyzed. The results are as follows: (1) The annual sunshine hours in the Qinghai Plateau exhibit a significant downward trend from 1961 to 2020, and a sudden decrease occurred in 2004. From the perspective of spatial distribution, the annual sunshine hours in other regions showed a significant decreasing trend except that the sunshine hours in some southern stations is flat or slightly increased. And the decreasing trend is most significant in Qaidam area and eastern agricultural area. (2) The decreasing trend of sunshine hours in high-latitude areas is significantly greater than that in low-latitude areas of the Qinghai Plateau. The variation trend of sunshine hours at different latitudes in spring is small. In summer and winter, the decreasing trend of sunshine hours at relatively high latitudes is significantly greater than that at low latitudes, and in autumn, the decreasing trend of sunshine hours at low and high latitudes is significantly greater than that at middle latitudes. (3) The decreasing trend of annual sunshine hours in relatively low-altitude areas of the Qinghai Plateau is significantly greater than that in relatively high-altitude areas. The change trend of sunshine hours at different altitudes in spring is small, flat, or slightly reduced. The decreasing trend of sunshine hours at relatively low altitudes is significantly greater than that at higher altitudes in summer and winter. The sunshine hours at different altitudes in autumn exhibit a decreasing trend, but there is a significant difference in the decreasing trend.
Subjects: Library Science，Information Science >> Information Science submitted time 2017-08-24
Abstract：虚拟现实技术近几年发展迅速，本文通过分析专利申请、专利权人、专利布局和热点技术等专利情报，为我国企业和相关科研机构进行技术研发和布局提供参考。
Peer Review Status:
Awaiting Review
Subjects: Biology >> Bioengineering submitted time 2017-06-01 Cooperative journals: 《中国生物工程杂志》
Abstract： Metabolic engineering in bacteria requires optimization of gene expression levels to balance metabolic flux distributions and minimize accumulation of toxic intermediates towards an efficient biosynthesis of target products. Small non-coding RNAs (sRNAs) in bacteria can interact with their target mRNA by base-pairing to inhibit or activate the translation of their target genes. Since small RNAs play critical regulatory roles in many physiological processes, they are considered to be powerful tools for the tuning of target genes expression in bacterial metabolic engineering. Many artificial sRNAs are applied in metabolic engineering in recent years. In this review, identification of sRNA targes genes, interaction between sRNAs and their targets and application of sRNAs in metabolic engineering were summarized.
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