Subjects: Biology >> Botany >> Applied botany submitted time 2019-02-25 Cooperative journals: 《广西植物》
Abstract：多刺绿绒蒿（Meconopsis horridula ）为罂粟科绿绒蒿属一年生草本植物，是一种极具观赏价值和药用价值的高山植物，目前处于濒危状态。研究多刺绿绒蒿种子的萌发特性对其种子育苗及人工栽培具有重要意义。该研究以多刺绿绒蒿的种子为材料，研究不同消毒剂、浸种时间、温度和外源植物激素对种子萌发特性的影响。结果表明：（1）最适消毒方法为 75%乙醇 1 min + 3% H2O2 5 min，最适浸种时间为 24 h，最适温度和光照条件为 20 ℃/10 ℃（光照 12 h/黑暗 12 h），用无菌水浸种后的种子发芽率为 49.67%。（2）GA3 100 ~ 600 mg·L-1 和 NAA 5 ~ 30 mg·L-1 可以提高种子的发芽率、发芽势和发芽指数，缩短发芽启动时间和发芽持续时间，对种子的萌发有促进作用。（3）6-BA 5 mg·L-1 和 10 mg·L-1 对种子的萌发有一定的促进作用，但不显著，6-BA 浓度≥15 mg·L-1 则抑制种子的萌发。（4）用 GA3 500 mg·L-1 浸种后的种子发芽指标最好，发芽率、发芽势和发芽指数分别为 69.67%、33.00%、4.51，种子的发芽起始时间和发芽持续时间分别为 10.67 d、11.67 d。
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-07-18 Cooperative journals: 《广西植物》
Abstract： Cymbidium tracyanum is a Orchidaceae species, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the flower industry. Plant tissue culture technology offers an effective tool for C. tracyanum large-scale production to avoid endangering its natural populations, yet there is a few research on the plant tissue culture of C. tracyanum. The current study evaluated the effects of basic medium and hormone combination on the plant regeneration of C. tracyanum. The effects of illumination time and culture temperature on the growth of plantlets were also studied. The results showed that the optimum basic medium for C. tracyanum growth was 1/2 MS. The optimal medium for protocorm induction was 1/2 MS＋1.0 mg·L-1 6-BA＋0.5 mg·L-1 NAA, 95.00% of the seeds developed into the protocorms after cultured for 50 d. The optimal medium for protocorm proliferation was 1/2 MS＋2.0 mg·L-1 NAA, the maximum proliferation multiple of protocorm reached 4.25 after cultured for 30 d. The optimal medium for protocorm differentiation was 1/2 MS＋2.0 mg· L-1 NAA＋60 g· L-1 mashed potato＋0.5 g· L-1acticarbon, the induction rate of indefinite bud was 98.33% after cultured for 10 d, the rooting rate was 94.67% after cultured for 40 d. In the process of plantlets subculture, temperature of 20℃ , illumination time of 12 h·d-1 and illumination intensity of 2 000 lx were suited for the growth of plantlets, the physiological scorch rate of leaf tip was 3.33%. Using humus as cultivation substrate, the transplanting survival rate of plantlets was 97.78%. This study provides an effective way for keeping good varieties of features and rapid propagation of C. tracyanum, at the same time helps to solve some theoretical problems in factory production of C. tracyanum.
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