Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-26 Cooperative journals: 《广西植物》
LIANG Huizi
YANG Jisheng
QIN Yi
PAN Weihu
XIAO Yufei
HUANG Ronglin
WANG Renjie
JIANG Yi
LIU Xiongsheng
Abstract： Hopea chinensis is distributed in China and its seeds are typical stubbornness. In order to investigate the germination adaptability of seeds to temperature and moisture, we carried out an experiment with three different constant temperature (15 ℃, 20 ℃ and 25 ℃) controlled by artificial climate incubators, and six polyethylene glycol (PEG-6000) mass percentage concentrations (0, 5%, 10%, 15%, 25% and 35%) were used to simulate drought stress. The germination characteristics of these seeds were carefully examined. The results were as follows: (1) Temperature exerted a significant influence on seed germination. Regardless of the level of drought stress applied, higher temperatures positively affected the germination rate, germination energy, germination index, radicle length, seeding length and vitality index of seeds, higher temperatures led to shorter germination delay and increased fluctuations in the germination period. (2) Drought stress had a significant impact on seed germination. Under identical temperature conditions, increasing levels of drought stress resulted in reduced germination rate, germination energy, germination index, germination period, radicle length, seeding length and vitality index of seeds. Moreover, higher levels of drought stress led to longer in germination delay of seeds. (3) The interaction between temperature and drought stress had a significant influenced on seed germination. Under drought stress, seed germination exhibited different responses under different temperature conditions. Specifically, under conditions where a PEG-6000 concentration of 35% was applied as drought stress treatment, the germination rates at temperatures of 20 °C and 25 °C were significantly higher than those observed at 15 °C (8.89% and 15.55% compared to lower rates at 15 °C). However, it was important to note that when subjected to both 35% PEG-6000 and a temperature of 15 °C, the seeds no longer germinate. In summary, our results indicate that the suitable temperatures for seed germination are identified as being around 20 °C and 25 °C. It is determined that the most favorable temperature for early seedling growth is 25 °C. As the drought stress intensifies, the inhibitory effect on seed germination become stronger. These findings suggest that temperature plays a positive role in promoting seed germination, while drought stress can significantly hinder this process. Additionally, moderate warming can help alleviate the inhibitory effect of drought stress on seed germination.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： Liquidambar formosana is an excellent landscape ecological tree species because its beautiful tree shape and red or orange leaves in autumn. In order to understand the genetic basis of discoloration and secondary metabolism of L. formosana leaves, the mixed samples of L. formosana leaves at five discoloration periods were used for full-length transcriptome sequencing using single-molecule real-time sequencing technique (PacBio platform). The results were as follows: (1) High-quality 41.04 Gb data were obtained by full-length transcriptome sequencing, from which 563 180 full-length non-chimeric sequences were identified, and 27 269 high-quality full-length transcripts were obtained by clustering and de-redundancy. In 27 269 full-length transcripts, 2 035 long-chain non-coding RNA (lncRNA) were predicted, and 14 892 simple repeat sequence (SSR) sites and 1 856 transcription factors were detected. (2) The results of gene annotation showed that a total of 24 857 transcripts were annotated in 8 databases such as NR, GO, COG and KEGG, and 124 metabolic pathways were obtained in KEGG database, including ribosome, carbon metabolism, amino acid biosynthesis and so on. 49 and 71 transcripts were involved in flavonoid and chlorophyll metabolism respectively. The above results preliminarily reveal the transcriptome information and functional characteristics of L. formosana leaves during the discoloration period, and provide basic data for the follow-up study of the molecular mechanism of discoloration, the pathway and regulation of pigment metabolism and synthesis, the cloning of related functional genes and the improvement of leaf color.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-11-27 Cooperative journals: 《广西植物》
Abstract：为探讨澳洲茶树组培苗耐受低温的能力及对低温胁迫的响应机制，该文测定了3月龄澳洲茶树组培苗低温处理过程及恢复培养后叶片叶绿素(Chl)、丙二醛(MDA)、抗氧化物酶(SOD, POD, CAT)、抗坏血酸(AsA)、谷胱甘肽(GSH)、可溶性糖(SS)、可溶性蛋白(SP)、游离脯氨酸(Pro)的变化。结果表明：(1)-5 ℃胁迫24 h，保护酶及抗氧化物质含量均显著下降，MDA含量显著上升。(2)0℃下，AsA和GSH先降后升，POD和CAT相反，SOD持续升高；胁迫48 h后保护酶活性显著升高，抗氧化剂含量显著降低。(3)10 ℃下，SOD活性先降后升；胁迫48 h后，渗透调节物质含量均显著升高。因此，3月龄澳洲茶树组培苗在-5 ℃受到致死冻害，幼苗能通过启动抗氧化系统及快速积累渗透调节物质响应0 ℃以上低温胁迫，但对10 ℃和0 ℃两种低温胁迫的应答机制却存在一定的差异。该研究结果将有助于深入了解其对低温的耐受能力及生理响应机制，为合理引种及规模化种植提供理论依据。
Subjects: Biology >> Bioengineering submitted time 2018-01-16 Cooperative journals: 《中国生物工程杂志》
Abstract：目的：利用烟草遗传转化体系，研究尾叶桉（Eucalyptus urophylla）咖啡酸氧甲基转移酶基因（EuCOMT）和咖啡酰CoA氧甲基转移酶（EuCCoAOMT）基因对木质素单体合成的定向调控效果。方法：分别利用EuCOMT的正义片段、EuCCoAOMT的全长RNAi片段进行单基因和二价基因的烟草转化研究，并对转基因烟草植株中目标基因表达水平、木质素和纤维素的含量、茎部解剖结构以及木质素单体含量进行检测。结果：分别获得了转基因植株C-S（转EuCOMT正义片段）、CR（转EuCCoAOMT全长RNAi片段）、C-CR（EuCOM和EuCCoAOMT二价基因转化）。烟草中转入的正义EuCOMT的片段能够正常表达，而EuCCoAOMT的全长RNAi片段对烟草CCoAOMT基因引发了强烈的抑制。转基因烟草的生长形态、木质素、纤维含量以及解剖结构与野生型无显著差异。转基因植株C-S中G木质素含量升高17.72%，S/G比值降低17.99%；CR中S/G比值升高61.62%，C-CR中G木质素降幅达到57.38%，S/G比值升幅达到114.94%。结论：抑制CCoAOMT对G木质素合成具有显著的抑制效果，EuCOMT和EuCCoAOMT二价基因转化对S/G比值的定向调控效果最为理想。
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