Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To explore the role of transient receptor potential canonical 1 (TRPC1) in airway remodeling and the effect of budesonide intervention on its expression in the lungs of guinea pigs with ovalbumin-induced asthma. Methods Fifty male guinea pigs were randomized into 5 equal groups, including a blank control group, ovalbumin group, ovalbumin+TRPC1 siRNA group, ovalbumin + luciferase siRNA group, and ovalbumin + budesonide group. After corresponding treatments, bronchoalveolar lavage was collected from the guinea pigs for eosinophils analysis and detection of IL-5 and IL-13 levels using ELISA. The lung tissues were stained with HE and Masson's trichrome to observe the bronchial wall thickness, smooth muscle hypertrophy, subepithelial collagen deposition, and lung inflammations. Immunohistochemistry and real-time quantitative PCR were performed to detect TRPC1 protein and mRNA expressions in the lungs, respectively. Results The guinea pig models of ovalbumin-induced asthma showed significantly increased thickness of the bronchial wall, smooth muscle hypertrophy, collagen deposition and inflammatory cell infiltration, but these pathologies were obviously alleviated by treatment with TRPC1 siRNA or budesonide (P<0.05). Immunohistochemstry showed that TRPC1 protein was distributed mainly on the cell membrane and in the nuclei of the basal cells or columnar epithelial cells. Conclusion The up-regulated expression of TRPC1 ion channel is closely associated with the occurrence and progression of airway remodeling and chronic airway inflammation in asthma. Budesonide can partially suppress airway remodeling and inflammation by regulating the expression of TRPC1.
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc. submitted time 2017-03-23
Abstract：摘要： 目的 介绍“中国彩巢计划：成长在中国（Chinese Color Nest Project – CCNP: Growing Up in China 2013-2022）”大型学龄儿童青少年脑与行为生长曲线项目。 方法 在全国范围内分期分步地开展毕生发展各年龄段的心理行为与脑影像样本积累，未来十年 CCNP 将基于加速纵向实验设计方法，建立中国人脑毕生发展的常模轨线。 结果 作为“彩巢”计划的脑发育项目，devCCNP 已经完成对重庆北碚区 192 名健康儿童青少年（6-18 岁）的 5 年追踪。 结论 devCCNP就实验设计、样本采集策略、数据获取和存储、初步结果和数据共享等方面都说明本计划具备长期实施的可行性。
Peer Review Status:
Awaiting Review
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