Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract: Expansin (EXP) plays an important role in plant response to environmental stress by regulating cell wall relaxation. To explore the role of EXP genes in soybean response to abiotic stress, two soybean EXP genes (GmEXPB5 and GmEXPB7) and their protein sequences were analyzed by bioinformatics, and their expression levels were detected by real-time fluorescent quantitative PCR (qRT-PCR). The results were as follows: (1) The GmEXPB5 and GmEXPB7 were located on chromosomes 10 and 12 of soybean, and encoded proteins containing 272 and 267 amino acids, respectively. The molecular weight of GmEXPB5 protein was 29.07 kD and the theoretical isoelectric point was 7.51. The molecular weight of GmEXPB7 protein was 29.09 kD and the theoretical isoelectric point was 8.66. Both GmEXPB5 and GmEXPB7 were stable hydrophilic proteins localized in the cell wall. Both GmEXPB5 and GmEXPB7 proteins contained a signal peptide sequence and a conserved DPBB_1 domain. (2) GmEXPB5 was closely related to CaEXPB15 of chickpea, and GmEXPB7 was closely related to EXPB3 of chickpea, red bean and cowpea. (3) GmEXPB5 and GmEXPB7 were expressed in soybean roots, stems and leaves, and their expression levels in roots and leaves were significantly higher than those in stems. (4) GmEXPB5 and GmEXPB7 could respond to salt, drought and cold stresses in soybean seedlings. (5) The promoter region of GmEXPB5 contained two types of stress-related cis-elements (ABRE and ARE). The promoter region of GmEXPB7 contained five types of stress-related cis-elements (ABRE、ARE、CGTCA-motif、TC-rich repeats and MBS). These results indicated that GmEXPB5 and GmEXPB7 could participate in the response of soybean to abiotic stress.
Subjects: Library Science,Information Science >> Library Science submitted time 2023-10-08 Cooperative journals: 《知识管理论坛》
Abstract: [Purpose/significance] With the development of internet and information technology, more and more social media have been begun to build a new business model of “community + e-commerce”. This paper aims to explore the user knowledge sharing on their consumer behavior in the content community, so as to provide a management strategy for improving the purchasing behavior of users in the community. [Method/ process] This article took “Little Red Book” as an example to analyze its internal operation mechanism, and based on Szulanski’s knowledge transfer model, built the influencing factor model of the knowledge sharing process on consumer purchase behavior in the “Little Red Book”. Finally,it took the users of the “Little Red Book” as the survey object, through the data of the questionnaire survey for empirical analysis. [Result/conclusion] In social media, knowledge sharing among users can be divided into four stages: knowledge generation, interaction, integration and feedback, in which user interaction, perceived risk and the professional ability of the recipient have positive impacts on their purchase behavior; The knowledge sharing process further influences the users’ willingness to purchase through the intermediary role of knowledge sharing content and degree, and the knowledge sharing content has a greater impact on it, which also indicates that in social media operations, enriching and improving community content and strengthen users, enhancing user interaction and reducing the perceived risk of users are the key to promoting user purchases.
Subjects: Astronomy >> Astrophysical processes submitted time 2019-07-30 Cooperative journals: 《天文研究与技术》
Abstract:经过十余年的不断建设和发展,中国虚拟天文台(China-VO)已成为支撑天文学观测、研究、教学的重要技术和资源平台。随着多信使天文学和时域天文学时代的到来,虚拟天文台也需要升级自身的核心能力,以给天文工作者提供更精准的服务和技术支撑。为此,中国虚拟天文台团队结合天文学的发展方向和信息技术发展趋势梳理了一份核心技术需求清单,并以问卷的形式针对领域内专家和用户开展了调研。通过对调研结果的统计和分析,中国虚拟天文台明确了未来一段时期的主要努力方向和目标,计划采用平台化的开发模式,并开放第三方开发接口,以吸引更多感兴趣的开发者基于虚拟天文台资源做出实用的工具,更好地实现资源与技术向服务的快速转换。
Subjects: Biology >> Zoology submitted time 2017-11-08 Cooperative journals: 《动物营养学报》
Abstract:为探究异地育肥牦牛模式,本试验对比了本地杂交肉牛(秦川×西门塔尔)在同等条件下血液生理生化指标和生长性能的差异
Subjects: Astronomy >> Astrophysical processes submitted time 2017-09-26 Cooperative journals: 《天文研究与技术》
Abstract:传统光学天象厅因为节目制作难度大,成本高昂,功能扩展困难等缺点逐渐被功能强大的数字天象厅取代。随着天文教育和科普的深入推广,国内对数字天象厅的需求越来越大。数字天象厅的核心是球幕投影技术。针对球幕投影技术存在的一些技术问题,如自动化程度不高,需要人工干预等,提出了面向中小型球幕天象厅易于实现的多投影自动拼接方案。对方案中的几何校正、亮度校正、颜色校正等核心功能进行了详细的论述。该方案便于操作,易于实现,对中小型球幕有良好表现,对大型球幕也有借鉴意义。
Subjects: Astronomy >> Astrophysical processes submitted time 2017-09-26 Cooperative journals: 《天文研究与技术》
Abstract:海量观测数据及次生数据的高效存储与检索,天文大数据的快速及时处理,加速天文学研究的科学产出等问题,已成为天文观测和天文研究迫切需要解决的难题。以信息技术为支撑的天文大数据的高效分析和处理,帮助天文学家重新审视和了解宇宙。虚拟天文台的出现为全球范围内研究资源的无缝透明连接提供了协议、标准,以协议为基拙规范了天文数据的发布与检索方式。以国内外现有的观测设备为基拙,综述目前主流天文机构的数据发布与检索相关情况。