submitted time 2024-01-11
Abstract： Objective To investigate the effects of Yangxue Roujin formula combined with aerobic exercise on inflammation and cartilage metabolism in a rabbit knee osteoarthritis (KOA) model by regulating the stromal cell derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) pathway. Methods New Zealand rabbits were used to establish a KOA model by injecting papain into the knee joint cavity. They were randomly separated into model group, Yangxue Roujin formula group, aerobic exercise group, Yangxue Roujin formula+aerobic exercise group, and celecoxib group, with 10 rabbits in each group. Another 10 New Zealand rabbits were collected as the control group. After grouping and intervention treatment, toluidine blue (TB) staining was applied to detect the pathological morphology of knee joint cartilage tissue of rabbits in each group and Mankin scoring was performed; scanning electron microscopy was applied to detect the morphology of knee joint cartilage in each group; immunohistochemical staining was applied to detect the expression of matrix metalloproteinase 13 (MMP13) and collagen type II (Col-II) in the cartilage tissue of rabbit knee joints in each group; enzyme linked immunosorbent assay (ELISA) was applied to measure the levels of inflammatory factors such as interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), IL-6, and IL-18 in the serum and knee joint fluid of rabbits in each group; immunoblotting was applied to detect the expression of SDF-1/CXCR4 pathway related proteins in rabbit knee joint cartilage tissue in each group. Results Compared with the control group, the rabbit knee joint cartilage in the model group was obviously damaged, the Mankin score and MMP13 positive expression in knee cartilage tissue, serum and knee joint fluid IL-17, TNF-α, IL-6, and IL-18 levels, and SDF-1 and CXCR4 protein expression in cartilage tissue were obviously increased (P<0.05), the Col-Ⅱ positive expression was obviously reduced (P<0.05); compared with the model group, the Yangxue Roujin formula group, aerobic exercise group, Yangxue Roujin formula+aerobic exercise group, and celecoxib group all showed reduced cartilage damage in the rabbit knee joint, the Mankin score and MMP13 positive expression in knee cartilage tissue, serum and knee joint fluid IL-17, TNF-α, IL-6, and IL-18 levels, and SDF-1 and CXCR4 protein expression in cartilage tissue were obviously reduced (P<0.05), the Col-Ⅱ positive expression was obviously increased (P<0.05); compared with the Yangxue Roujin formula group and the aerobic exercise group, the Yangxue Roujin formula+aerobic exercise group and the Celecoxib group all showed a reduction in rabbit knee joint cartilage damage, the Mankin score and MMP13 positive expression in knee cartilage tissue, serum and knee joint fluid IL-17, TNF-α, IL-6, and IL-18 levels, and SDF-1 and CXCR4 protein expression in cartilage tissue were obviously reduced (P<0.05), the Col-Ⅱ positive expression was obviously increased (P<0.05). Conclusion The Yangxue Roujin formula and aerobic exercise can down-regulate the expression of SDF-1/CXCR4 pathway proteins, inhibit the release of inflammatory factors, thereby reducing knee joint inflammation in KOA rabbits, improving cartilage metabolism, alleviating collagen degradation and tissue degeneration in knee joint cartilage. The two have a synergistic effect.
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Subjects: Biology >> Botany >> Applied botany submitted time 2020-03-24 Cooperative journals: 《广西植物》
Abstract：木麻黄海防林是海南岛重要的海岸生态屏障，天然更新对其持续发挥防护功能具有重要意义。调查发现海南岛大多数木麻黄林林下天然更新困难，然而却存在局部更新良好的现象。为了探究天然更新存在巨大差异的原因，通过分析不同林地更新质量的差异，研究影响木麻黄海防林林下天然更新的主要影响因子，为促进海南海防林由人工林向近自然林转化提供一定的理论基础。本研究在海南岛木麻黄海防林中共设置73块临时样地，采用方差分析和相关分析等统计方法，分别研究林地所属气候区、林分条件、土壤因子和凋落物累积量对天然更新质量和密度的影响。结果表明：湿润气候区的木麻黄林下更新要显著优于半干旱区；木麻黄林分密度与更新密度和草本盖度存在显著负相关，但林分条件其它因子对更新影响不大；不同更新质量样地的土壤pH和养分均无显著性差异，但铵态氮对幼苗、有机质对幼树的更新存在一定的促进作用；凋落物的累计整体不利于天然更新的进行。结果说明气候因子，木麻黄林分密度，木麻黄凋落物积累量是影响木麻黄海防林林下植物天然更新的主要因素。
Subjects: Biology >> Botany >> Applied botany submitted time 2020-03-24 Cooperative journals: 《广西植物》
Abstract：成功的天然更新应同时具备三个条件，即（1）种源数量充足、质量良好；（2）适宜种子萌发的微生境；（3）幼苗、幼树存活的生态条件。为探究海南岛木麻黄（Casuarina equisetifolia）海防林自身无法天然更新的障碍因子，对影响其天然更新的三个条件之一的种子萌发条件进行了研究，探讨不同的生态因子，如：木麻黄化感，土壤酸碱度、盐度，温度，基质类型，水分等对木麻黄种子萌发的影响。研究结果表明：不同浸提物的不同浸提液浓度处理的种子萌发率与CK组无显著性差异；设定范围内的pH、盐度和温度对木麻黄种子萌发率无显著影响；不同浓度梯度PEG溶液处理的木麻黄种子萌发率存在显著性差异，且伴随PEG溶液浓度增加，木麻黄种子萌发率随之锐减；不同基质及浇水频度对种子萌发率也具有显著影响。综合PEG干旱胁迫，基质及浇水频度的结果可以发现，木麻黄种子抗旱能力较弱，对水分敏感，因此，水分是制约木麻黄种子萌发的主要限制因子，同时凋落物层及滨海沙土较差的保水性也不同程度制约了种子的萌发。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-02-25 Cooperative journals: 《广西植物》
Abstract：天然更新是森林资源再生产的一个重要的生态学过程，良好的天然更新可促进人工林向近自然林方向的转变。为了解海南木麻黄海防林天然更新现状及特征，并筛选出自然条件下天然更新良好的树种，该研究采用典型抽样的调查方法对海南全岛木麻黄海防林天然更新状况进行了系统调查，并应用天然更新密度及物种丰富度等指标，对干湿不同气候区木麻黄天然更新特征进行了比较分析；应用更新密度、更新指数等指标对天然更新质量较好的树种进行了筛选。在海南岛木麻黄海防林内共计设立临时样地 73 块，样地总面积为 2.51 hm2。结果表明：（1）海南岛木麻黄海防林下天然更新质量整体不佳，木麻黄自身无法实现天然更新，但局部其他树种天然更新良好，更新良好的比例为 15.1%；（2）湿润区天然更新丰富度及物种多样性远优于半干旱区，其中湿润区样地更新树种为 28 科，50 属，59 种；半干旱区样地更新的树种仅为 6 科，6 属，6 种；（3）乔木更新情况优于灌木；（4）潺槁木姜子、鸦胆子、台湾相思、榄仁树等具有较强的天然更新性能，适合与木麻黄混交，可作为木麻黄海防林的混交树种或伴生树种。
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